ISDA: 9S5N

Deposition Date 2025-07-29

Release Date 2026-04-08

Last Version Date 2026-04-08

Entry Detail

PDB ID:

9S5N

Keywords:

CELL CYCLE

Title:

Cryo-EM structure of the Saccharomyces cerevisiae KMN junction complex containing the Mis12c(Mtw1c) head 2 domain

Biological Source:

Source Organism(s):

Saccharomyces cerevisiae (Taxon ID: 4932)
Saccharomyces cerevisiae S288C (Taxon ID: 559292)

Expression System(s):

Trichoplusia ni

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

7.20 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

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Macromolecular Entities

Polymer Type:polypeptide(L)
Molecule:Kinetochore protein SPC24
Chain IDs:F (auth: 24)
Chain Length:213
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

Polymer Type:polypeptide(L)
Molecule:Kinetochore protein SPC25
Chain IDs:G (auth: 25)
Chain Length:221
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

Polymer Type:polypeptide(L)
Molecule:Kinetochore-associated protei
Chain IDs:E (auth: Ds)
Chain Length:576
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

Polymer Type:polypeptide(L)
Molecule:Outer kinetochore KNL1 comple
Chain IDs:D (auth: Kl)
Chain Length:474
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

Polymer Type:polypeptide(L)
Molecule:Kinetochore-associated protei
Chain IDs:B (auth: Mt)
Chain Length:289
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

Polymer Type:polypeptide(L)
Molecule:Kinetochore-associated protei
Chain IDs:A (auth: Nn)
Chain Length:201
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae

Polymer Type:polypeptide(L)
Molecule:Kinetochore-associated protei
Chain IDs:H (auth: Ns)
Chain Length:216
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

Polymer Type:polypeptide(L)
Molecule:Outer kinetochore KNL1 comple
Chain IDs:C (auth: Zw)
Chain Length:420
Number of Molecules:1
Biological Source:Saccharomyces cerevisiae S288C

Ligand Molecules

Primary Citation

Assembly and phospho-regulatory mechanisms of the budding yeast outer kinetochore KMN complex

Turner, N.N, Zhang, Z, Muir, K.W, McLaughlin, S.H, Morgan, T, Barford, D.Show

To Be Published ? ? ? (?)

Primary Citation of related structures

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