9RHU image
Deposition Date 2025-06-10
Release Date 2026-01-21
Last Version Date 2026-02-18
Entry Detail
PDB ID:
9RHU
Keywords:
RIBOSOME
Title:
Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop codon in mutated F2A sequence
Biological Source:
Source Organism(s):
Homo sapiens (Taxon ID: 9606)
Foot-and-mouth disease virus SAT 2 (Taxon ID: 35292)
Oryctolagus cuniculus (Taxon ID: 9986)
Expression System(s):
Oryctolagus cuniculus, Escherichia coli bl21(de3)
Method Details:
Experimental Method:
ELECTRON MICROSCOPY
Resolution:
2.65 Å
Aggregation State:
PARTICLE
Reconstruction Method:
SINGLE PARTICLE
9RHU validation report
Macromolecular Entities
Polymer Type:polyribonucleotide
Molecule:18S rRNA
Chain IDs:B (auth: A1)
Chain Length:145
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L6
Gene (Uniprot):RPL6
Chain IDs:C (auth: A2)
Chain Length:135
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1SKF7 Pfam ID:PF03868, PF01159
Polymer Type:polyribonucleotide
Molecule:28S rRNA
Chain IDs:D (auth: A3)
Chain Length:4808
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Small ribosomal subunit prote
Gene (Uniprot):RPS27
Chain IDs:E (auth: B1)
Chain Length:84
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1TZ76 Pfam ID:PF01667
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L7
Gene (Uniprot):RPL7
Chain IDs:F (auth: B2)
Chain Length:249
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1SV32 Pfam ID:PF08079, PF00327
Polymer Type:polyribonucleotide
Molecule:5S rRNA
Chain IDs:G (auth: B3)
Chain Length:119
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Small ribosomal subunit prote
Gene (Uniprot):RPS28
Chain IDs:H (auth: C1)
Chain Length:69
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1TIB4 Pfam ID:PF01200
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):RPL7A
Chain IDs:I (auth: C2)
Chain Length:319
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1STW0 Pfam ID:PF01248
Polymer Type:polyribonucleotide
Molecule:5.8S rRNA
Chain IDs:J (auth: C3)
Chain Length:158
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Ubiquitin-ribosomal protein e
Gene (Uniprot):RPS27A
Chain IDs:K (auth: D1)
Chain Length:80
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1SK22 Pfam ID:PF01599
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L9
Gene (Uniprot):RPL9
Chain IDs:L (auth: D2)
Chain Length:123
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1SWI6 Pfam ID:PF00347
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):RPL8
Chain IDs:M (auth: D3)
Chain Length:257
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1TT27 Pfam ID:PF00181, PF03947
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein eS30
Gene (Uniprot):FAU
Chain IDs:N (auth: E1)
Chain Length:59
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1T8A2 Pfam ID:PF04758
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L10
Gene (Uniprot):RPL10
Chain IDs:O (auth: E2)
Chain Length:214
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:B7NZQ2 Pfam ID:PF00252
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Ribosomal protein L3
Gene (Uniprot):RPL3
Chain IDs:P (auth: E3)
Chain Length:403
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1TL06 Pfam ID:PF00297
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:eS26
Chain IDs:Q (auth: F1)
Chain Length:119
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:A0A2K5LQY6 Pfam ID:PF01283
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L11
Gene (Uniprot):RPL11
Chain IDs:R (auth: F2)
Chain Length:178
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1TUB8 Pfam ID:PF00281, PF00673
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):RPL4
Chain IDs:S (auth: F3)
Chain Length:413
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1SVW5 Pfam ID:PF00573, PF14374
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Small ribosomal subunit prote
Gene (Uniprot):RACK1
Chain IDs:T (auth: G1)
Chain Length:84
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1SJB4 Pfam ID:PF00400
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Choriogonadotropin subunit be
Chain IDs:U (auth: G2)
Chain Length:70
Number of Molecules:1
Biological Source:Homo sapiens, Foot-and-mouth disease virus SAT 2
UniProt ID:Q9Q2N9
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):RPL5
Chain IDs:V (auth: G3)
Chain Length:296
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:P19949 Pfam ID:PF17144, PF14204
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Small ribosomal subunit prote
Gene (Uniprot):RPS29
Chain IDs:W (auth: H1)
Chain Length:130
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1U7M4 Pfam ID:PF00253
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):RPL13
Chain IDs:X (auth: H2)
Chain Length:211
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1TKB3 Pfam ID:PF01294
Polymer Type:polyribonucleotide
Molecule:mRNA containing F2A sequence
Chain IDs:Y (auth: I1)
Chain Length:659
Number of Molecules:1
Biological Source:Foot-and-mouth disease virus SAT 2
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L14
Gene (Uniprot):RPL14
Chain IDs:Z (auth: I2)
Chain Length:52
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1SZ12 Pfam ID:PF01929
Polymer Type:polyribonucleotide
Molecule:E-site tRNA-Pro
Chain IDs:AA (auth: J1)
Chain Length:133
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Ribosomal protein L15
Gene (Uniprot):RPL15
Chain IDs:BA (auth: J2)
Chain Length:204
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1T0C1 Pfam ID:PF00827
Polymer Type:polyribonucleotide
Molecule:P-site tRNA-Gly
Chain IDs:CA (auth: K1)
Chain Length:124
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):RPL13A
Chain IDs:DA (auth: K2)
Chain Length:92
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1TVS8 Pfam ID:PF00572
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Small ribosomal subunit prote
Gene (Uniprot):RPSA
Chain IDs:EA (auth: L1)
Chain Length:295
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1TLT8 Pfam ID:PF00318, PF16122
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):RPL17
Chain IDs:FA (auth: L2)
Chain Length:137
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1SCJ6 Pfam ID:PF00237
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S3a
Gene (Uniprot):RPS3A
Chain IDs:GA (auth: M1)
Chain Length:264
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1SS70 Pfam ID:PF01015
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:eL18
Gene (Uniprot):RPL18
Chain IDs:HA (auth: M2)
Chain Length:188
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:F6QKI9 Pfam ID:PF17135
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L34
Gene (Uniprot):RPL34
Chain IDs:A (auth: MB)
Chain Length:117
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1TXG5 Pfam ID:PF01199
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S2
Gene (Uniprot):RPS2
Chain IDs:IA (auth: N1)
Chain Length:293
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:O18789 Pfam ID:PF00333, PF03719
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Ribosomal protein L19
Gene (Uniprot):RPL19
Chain IDs:JA (auth: N2)
Chain Length:165
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1TYL6 Pfam ID:PF01280, PF25476
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Small ribosomal subunit prote
Gene (Uniprot):RPS3
Chain IDs:KA (auth: O1)
Chain Length:243
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1TNM3 Pfam ID:PF07650, PF00189
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):RPL18A
Chain IDs:LA (auth: O2)
Chain Length:217
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1TTY7 Pfam ID:PF01775
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S4
Gene (Uniprot):RPS4X
Chain IDs:MA (auth: P1)
Chain Length:263
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1TK17 Pfam ID:PF08071, PF00900, PF00467, PF16121
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L21
Gene (Uniprot):RPL21
Chain IDs:NA (auth: P2)
Chain Length:438
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1SHQ2 Pfam ID:PF01157
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Small ribosomal subunit prote
Gene (Uniprot):RPS5
Chain IDs:OA (auth: Q1)
Chain Length:204
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1TFM5 Pfam ID:PF00177
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L22
Gene (Uniprot):RPL22
Chain IDs:PA (auth: Q2)
Chain Length:128
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1TSG1 Pfam ID:PF01776
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S6
Gene (Uniprot):RPS6
Chain IDs:QA (auth: R1)
Chain Length:249
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1TM55 Pfam ID:PF01092
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Ribosomal protein L23
Gene (Uniprot):RPL23
Chain IDs:RA (auth: R2)
Chain Length:140
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1T6D1 Pfam ID:PF00238
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Small ribosomal subunit prote
Gene (Uniprot):RPS7
Chain IDs:SA (auth: S1)
Chain Length:194
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1SVB0 Pfam ID:PF01251
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Ribosomal protein L24
Gene (Uniprot):RPL24
Chain IDs:TA (auth: S2)
Chain Length:157
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1SE28 Pfam ID:PF01246
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S8
Gene (Uniprot):RPS8
Chain IDs:UA (auth: T1)
Chain Length:208
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1TJW1 Pfam ID:PF01201
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):RPL23A
Chain IDs:VA (auth: T2)
Chain Length:156
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1SE76 Pfam ID:PF03939, PF00276
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Small ribosomal subunit prote
Gene (Uniprot):RPS9
Chain IDs:WA (auth: U1)
Chain Length:194
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:B7NZS8 Pfam ID:PF00163, PF01479
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Ribosomal protein L26
Gene (Uniprot):RPL26
Chain IDs:XA (auth: U2)
Chain Length:145
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1SQH0 Pfam ID:PF16906, PF00467
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:eS10
Gene (Uniprot):RPS10
Chain IDs:YA (auth: V1)
Chain Length:165
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1T168 Pfam ID:PF03501
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L27
Gene (Uniprot):RPL27
Chain IDs:ZA (auth: V2)
Chain Length:136
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1TXF6 Pfam ID:PF00467, PF01777
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Small ribosomal subunit prote
Gene (Uniprot):RPS11
Chain IDs:AB (auth: W1)
Chain Length:158
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1TRM4 Pfam ID:PF16205, PF00366
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L27a
Gene (Uniprot):RPL27A
Chain IDs:BB (auth: W2)
Chain Length:148
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1SNY0 Pfam ID:PF00828
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Small ribosomal subunit prote
Gene (Uniprot):RPS12
Chain IDs:CB (auth: X1)
Chain Length:132
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1SFR8 Pfam ID:PF01248
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L29
Gene (Uniprot):RPL29
Chain IDs:DB (auth: X2)
Chain Length:245
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1SGR6 Pfam ID:PF01779
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Small ribosomal subunit prote
Gene (Uniprot):RPS13
Chain IDs:EB (auth: Y1)
Chain Length:151
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1SP51 Pfam ID:PF08069, PF00312
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L30
Gene (Uniprot):RPL30
Chain IDs:FB (auth: Y2)
Chain Length:115
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1TDL2 Pfam ID:PF01248
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Small ribosomal subunit prote
Gene (Uniprot):RPS14
Chain IDs:GB (auth: Z1)
Chain Length:151
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1T1F0 Pfam ID:PF00411
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L31
Gene (Uniprot):RPL31
Chain IDs:HB (auth: Z2)
Chain Length:125
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1SHG0 Pfam ID:PF01198
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Small ribosomal subunit prote
Gene (Uniprot):RPS15
Chain IDs:IB (auth: a1)
Chain Length:145
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1U0Q2 Pfam ID:PF00203
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Ribosomal protein L32
Chain IDs:JB (auth: a2)
Chain Length:135
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:A0AAA9WVN6 Pfam ID:PF01655
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Small ribosomal subunit prote
Gene (Uniprot):RPS16
Chain IDs:KB (auth: b1)
Chain Length:84
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1SGX4 Pfam ID:PF00380
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L35a
Gene (Uniprot):RPL35A
Chain IDs:LB (auth: b2)
Chain Length:249
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1SF08 Pfam ID:PF01247
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Small ribosomal subunit prote
Gene (Uniprot):RPS17
Chain IDs:MB (auth: c1)
Chain Length:69
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1TU13 Pfam ID:PF00833
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Small ribosomal subunit prote
Gene (Uniprot):RPS18
Chain IDs:NB (auth: d1)
Chain Length:80
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1TPG3 Pfam ID:PF00416
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L35
Gene (Uniprot):RPL35
Chain IDs:OB (auth: d2)
Chain Length:123
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1SIT5 Pfam ID:PF00831
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein eS19
Chain IDs:PB (auth: e1)
Chain Length:59
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:A0ABF7PQ95
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):RPL36
Chain IDs:QB (auth: e2)
Chain Length:214
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1TTQ5 Pfam ID:PF01158
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Small ribosomal subunit prote
Gene (Uniprot):RPS20
Chain IDs:RB (auth: f1)
Chain Length:119
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1SIZ2 Pfam ID:PF00338
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Ribosomal protein L37
Gene (Uniprot):RPL37
Chain IDs:SB (auth: f2)
Chain Length:178
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:U3KPD5 Pfam ID:PF01907
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Small ribosomal subunit prote
Gene (Uniprot):RPS21
Chain IDs:TB (auth: g1)
Chain Length:84
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1TM82 Pfam ID:PF01249
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L38
Gene (Uniprot):RPL38
Chain IDs:UB (auth: g2)
Chain Length:70
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1U001 Pfam ID:PF01781
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Small ribosomal subunit prote
Gene (Uniprot):RPS15A
Chain IDs:VB (auth: h1)
Chain Length:130
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1TG89 Pfam ID:PF00410
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L39-lik
Gene (Uniprot):LOC100343258
Chain IDs:WB (auth: h2)
Chain Length:211
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1TTN1 Pfam ID:PF00832
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S23
Gene (Uniprot):RPS23
Chain IDs:XB (auth: i1)
Chain Length:659
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1SZ47 Pfam ID:PF00164
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Ubiquitin-ribosomal protein e
Gene (Uniprot):UBA52
Chain IDs:YB (auth: i2)
Chain Length:52
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:P0DXC2 Pfam ID:PF01020
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S24
Gene (Uniprot):RPS24
Chain IDs:ZB (auth: j1)
Chain Length:133
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:A0A5F9D2E6 Pfam ID:PF01282
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Ribosomal protein L36a
Chain IDs:AC (auth: j2)
Chain Length:204
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:A0A5F9D391 Pfam ID:PF00935
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Small ribosomal subunit prote
Gene (Uniprot):RPS25
Chain IDs:BC (auth: k1)
Chain Length:124
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1TDB3 Pfam ID:PF03297
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L37a
Gene (Uniprot):RPL37A
Chain IDs:CC (auth: k2)
Chain Length:92
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1SY53 Pfam ID:PF01780
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Small ribosomal subunit prote
Gene (Uniprot):RPL41
Chain IDs:DC (auth: l1)
Chain Length:295
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:A0A087WNH4 Pfam ID:PF05162
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:[histone H4]-N-methyl-L-lysin
Gene (Uniprot):KMT5C
Chain IDs:EC (auth: l2)
Chain Length:137
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:A0A8C0DF35
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Large ribosomal subunit prote
Gene (Uniprot):RPLP0
Chain IDs:FC (auth: m2)
Chain Length:188
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1SPK4 Pfam ID:PF00466, PF17777, PF00428
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:60S ribosomal protein L12
Gene (Uniprot):RPL12
Chain IDs:GC (auth: n2)
Chain Length:165
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1SMR7 Pfam ID:PF03946, PF00298
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Ribosomal protein uL1
Gene (Uniprot):RPL10A
Chain IDs:HC (auth: o2)
Chain Length:217
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1SKZ8 Pfam ID:PF00687
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Eukaryotic peptide chain rele
Gene (Uniprot):ETF1
Mutagens:G183A, G184A
Chain IDs:IC (auth: p2)
Chain Length:438
Number of Molecules:1
Biological Source:Homo sapiens
UniProt ID:P62495 Pfam ID:PF03463, PF03464, PF03465
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:ATP binding cassette subfamil
Gene (Uniprot):ABCE1
Chain IDs:JC (auth: q2)
Chain Length:128
Number of Molecules:1
Biological Source:Oryctolagus cuniculus
UniProt ID:G1SG72 Pfam ID:PF04068, PF00037, PF00005
Modified Residue
Compound ID Chain ID Parent Comp ID Details 2D Image
1MA D A modified residue
5MC D C modified residue
6MZ D A modified residue
A2M D A modified residue
HIC P HIS modified residue
HY3 XB PRO modified residue
M3L YB LYS modified residue
MLZ DB LYS modified residue
NMM PB ARG modified residue
OMC D C modified residue
OMG D G modified residue
OMU D U modified residue
PSU D U modified residue
UR3 D U modified residue
V5N M HIS modified residue
Ligand Molecules
GTP
K
MG
SF4
SPD
ZN
Primary Citation
Molecular architecture and diversity of StopGo/2A translational recoding.
Proc.Natl.Acad.Sci.USA 123 e2528667123 e2528667123 (2026)
PMID: 41576085 DOI: 10.1073/pnas.2528667123

Abstact

Viral 2A sequences trigger a cotranslational peptide bond formation "skipping" event, termed "StopGo," to generate two separate proteins from a single open reading frame without classical termination. To investigate the mechanism of StopGo, we determined the cryo-EM structure of a mammalian ribosome positioned at the foot-and-mouth disease virus 2A (F2A) site. The structure shows how interactions between the F2A nascent chain (NC) and the ribosomal exit tunnel induce a conformational change in the peptidyl transferase center that precludes further translation elongation but instead pre-exposes the P-tRNA:F2A-NC ester bond for hydrolysis and NC release. Additionally, we bioinformatically characterized variation and host association across nearly 10,000 StopGo sequences identified in virus genomes. We expanded the canonical core motif to (D/G/C/N)(V/I)ExNPGP and identified additional rare but functional variants. We also revealed several distinct upstream motifs that we showed biochemically to be important for StopGo activity. Interestingly, although StopGo is known to be functionally active in plants, we found no evidence for natural utilization of StopGo by plant viruses. Overall, these findings provide valuable insights into a unique translation recoding mechanism, and lay foundations for further optimization of multigene expression in biotechnology.

Legend

Protein

Chemical

Disease

Primary Citation of related structures
9RHU
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