ISDA: 9RAF

Deposition Date 2025-05-20

Release Date 2026-03-11

Last Version Date 2026-03-25

Entry Detail

PDB ID:

9RAF

Keywords:

VIRAL PROTEIN

Title:

Influenza A/H7N9 polymerase in complex with a 70-mer template in stalled elongation with backtracking and stem.

Biological Source:

Source Organism(s):

Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)) (Taxon ID: 1318616)
Homo sapiens (Taxon ID: 9606)

Expression System(s):

Trichoplusia ni

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

2.15 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

Download Validation Report

Macromolecular Entities

Polymer Type:polypeptide(L)
Molecule:Polymerase acidic protein
Mutagens:E349K; R490I
Chain IDs:A
Chain Length:716
Number of Molecules:1
Biological Source:Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))

Polymer Type:polypeptide(L)
Molecule:RNA-directed RNA polymerase c
Mutagens:K577G
Chain IDs:E (auth: B)
Chain Length:757
Number of Molecules:1
Biological Source:Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))

Polymer Type:polypeptide(L)
Molecule:Polymerase basic protein 2
Mutagens:G74R; K627; D701
Chain IDs:B (auth: C)
Chain Length:788
Number of Molecules:1
Biological Source:Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))

Polymer Type:polypeptide(L)
Molecule:RNA Pol II CTD 6 repeats (sit
Chain IDs:C (auth: G)
Chain Length:42
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polyribonucleotide
Molecule:RNA product
Chain IDs:D (auth: M)
Chain Length:13
Number of Molecules:1
Biological Source:Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))

Polymer Type:polyribonucleotide
Molecule:RNA template (70-mer)
Chain IDs:F (auth: T)
Chain Length:70
Number of Molecules:1
Biological Source:Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))

Ligand Molecules

Primary Citation

Polymerase trapping as the mechanism of H5 highly pathogenic avian influenza virus genesis.

Funk, M, Spronken, M.I, Hutchinson, R.M, Arragain, B, Juyoux, P, Bestebroer, T.M, de Bruin, A.C.M, Gultyaev, A.P, Fouchier, R.A.M, Cusack, S, Te Velthuis, A.J.W, Richard, M.Show

Science 391 eadr6632 eadr6632 (2026)

PMID: 41818353 DOI: 10.1126/science.adr6632

Primary Citation of related structures

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