ISDA: 9QJ1

Deposition Date 2025-03-18

Release Date 2026-04-08

Last Version Date 2026-04-08

Entry Detail

PDB ID:

9QJ1

Keywords:

HYDROLASE

Title:

Crystal structure of S-adenosyl-L-homocysteine hydrolase from P. aeruginosa, Q65N mutant soaked with adenosine and probed with rubidium to confirm disruption of a potassium binding site.

Biological Source:

Source Organism(s):

Pseudomonas aeruginosa PAO1 (Taxon ID: 208964)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

2.34 Å

R-Value Free:

0.20

R-Value Work:

0.18

R-Value Observed:

0.22

Space Group:

P 1 21 1

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Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Adenosylhomocysteinase
Gene (Uniprot):ahcY
Chain IDs:A, B, C, D, E (auth: H), F (auth: I), G (auth: J), H (auth: K)
Chain Length:472
Number of Molecules:8
Biological Source:Pseudomonas aeruginosa PAO1

UniProt ID:Q9I685 Pfam ID:PF05221, PF00670 EC:3.3.1.1

Ligand Molecules

ADN

NAD

PO4

Primary Citation

Crystal structure of S-adenosyl-L-homocysteine hydrolase from P. aeruginosa, Q65N mutant soaked with adenosine and probed with rubidium to confirm disruption of a potassium binding site.

Malecki, P.H, Wozniak, K, Stepniewska, M, Brzezinski, K, Liebschner, D, Afonine, P.V, Baker, M.L, Bunkoczi, G, Chen, V.B, Croll, T.I, Hintze, B, Hung, L.W, Jain, S, McCoy, A.J, Moriarty, N.W, Oeffner, R.D, Poon, B.K, Prisant, M.G, Read, R.J, Richardson, J.S, Richardson, D.C, Sammito, M.D, Sobolev, O.V, Stockwell, D.H, Terwilliger, T.C, Urzhumtsev, A.G, Videau, L.L, Williams, C.J, Adams, P.D.Show

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Primary Citation of related structures

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