ISDA: 9NZS

Deposition Date 2025-04-01

Release Date 2026-04-08

Last Version Date 2026-04-08

Entry Detail

PDB ID:

9NZS

Keywords:

RNA BINDING PROTEIN/DNA/RNA

Title:

Cryo-EM structure of OsCas12f-gRNA-DNA ternary complex. State III

Biological Source:

Source Organism(s):

Oscillibacter sp. (Taxon ID: 1945593)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

3.68 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

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Macromolecular Entities

Polymer Type:polypeptide(L)
Molecule:Cas12f
Chain IDs:A, B
Chain Length:433
Number of Molecules:2
Biological Source:Oscillibacter sp.

Polymer Type:polydeoxyribonucleotide
Molecule:Targer strand DNA
Chain IDs:C
Chain Length:45
Number of Molecules:1
Biological Source:Oscillibacter sp.

Polymer Type:polydeoxyribonucleotide
Molecule:Non-target strand DNA
Chain IDs:D
Chain Length:45
Number of Molecules:1
Biological Source:Oscillibacter sp.

Polymer Type:polyribonucleotide
Molecule:guide RNA
Chain IDs:E
Chain Length:151
Number of Molecules:1
Biological Source:Oscillibacter sp.

Polymer Type:polydeoxyribonucleotide
Molecule:DNA target poly C
Chain IDs:F (auth: G)
Chain Length:4
Number of Molecules:1
Biological Source:Oscillibacter sp.

Ligand Molecules

Primary Citation

Cryo-EM structure of CRISPR-Cas12f nucleases

Guan, K, Ocampo, R.F, Taylor, D.W.Show

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