ISDA: 9NZO

Deposition Date 2025-04-01

Release Date 2026-04-08

Last Version Date 2026-04-08

Entry Detail

PDB ID:

9NZO

Keywords:

DNA BINDING PROTEIN/DNA/RNA

Title:

Structure of Al3Cas12f State I

Biological Source:

Source Organism(s):

Escherichia coli (Taxon ID: 562)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

3.20 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

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Macromolecular Entities

Polymer Type:polypeptide(L)
Molecule:Cas12f Effector
Chain IDs:A (auth: B), E (auth: A)
Chain Length:506
Number of Molecules:2
Biological Source:Escherichia coli

Polymer Type:polyribonucleotide
Molecule:sgRNA
Chain IDs:D (auth: C)
Chain Length:154
Number of Molecules:1
Biological Source:Escherichia coli

Polymer Type:polydeoxyribonucleotide
Molecule:Target Strand DNA
Chain IDs:B (auth: D)
Chain Length:50
Number of Molecules:1
Biological Source:Escherichia coli

Polymer Type:polydeoxyribonucleotide
Molecule:Non-target Strand DNA
Chain IDs:C (auth: E)
Chain Length:50
Number of Molecules:1
Biological Source:Escherichia coli

Ligand Molecules

Primary Citation

MetagenCas12f Intermediate Structure

Fregoso Ocampo, R, Guan, K, Taylor, D.W.Show

To Be Published ? ? ? (?)

Primary Citation of related structures

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