ISDA: 9MBQ

Deposition Date 2025-03-17

Release Date 2026-03-25

Last Version Date 2026-04-08

Entry Detail

PDB ID:

9MBQ

Keywords:

HYDROLASE

Title:

Substrate-engaged human 26S proteasome bound to midnolin with RPT5 at top of spiral staircase

Biological Source:

Source Organism(s):

Homo sapiens (Taxon ID: 9606)

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

3.08 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

Download Validation Report

Macromolecular Entities

Polymer Type:polypeptide(L)
Molecule:26S proteasome regulatory sub
Chain IDs:L (auth: A)
Chain Length:433
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:26S proteasome regulatory sub
Chain IDs:M (auth: B)
Chain Length:377
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:26S proteasome regulatory sub
Chain IDs:N (auth: C)
Chain Length:310
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:26S proteasome regulatory sub
Chain IDs:O (auth: D)
Chain Length:418
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:26S proteasome regulatory sub
Chain IDs:P (auth: E)
Chain Length:389
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:26S proteasome regulatory sub
Chain IDs:Q (auth: F)
Chain Length:468
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:Proteasome subunit alpha type
Chain IDs:R (auth: G), FA (auth: g)
Chain Length:245
Number of Molecules:2
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:Proteasome subunit alpha type
Chain IDs:S (auth: H), GA (auth: h)
Chain Length:233
Number of Molecules:2
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:Proteasome subunit alpha type
Chain IDs:T (auth: I), HA (auth: i)
Chain Length:260
Number of Molecules:2
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:Proteasome subunit alpha type
Chain IDs:U (auth: J), IA (auth: j)
Chain Length:247
Number of Molecules:2
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:Proteasome subunit alpha type
Chain IDs:V (auth: K), JA (auth: k)
Chain Length:240
Number of Molecules:2
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:Isoform Long of Proteasome su
Chain IDs:W (auth: L), KA (auth: l)
Chain Length:268
Number of Molecules:2
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:Proteasome subunit alpha type
Chain IDs:X (auth: M), LA (auth: m)
Chain Length:254
Number of Molecules:2
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:Proteasome subunit beta type-
Chain IDs:Y (auth: N), MA (auth: n)
Chain Length:238
Number of Molecules:2
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:Proteasome subunit beta type-
Chain IDs:Z (auth: O), NA (auth: o)
Chain Length:276
Number of Molecules:2
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:Proteasome subunit beta type-
Chain IDs:AA (auth: P), OA (auth: p)
Chain Length:204
Number of Molecules:2
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:Proteasome subunit beta type-
Chain IDs:BA (auth: Q), PA (auth: q)
Chain Length:201
Number of Molecules:2
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:Proteasome subunit beta type-
Chain IDs:CA (auth: R), QA (auth: r)
Chain Length:262
Number of Molecules:2
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:Proteasome subunit beta type-
Chain IDs:DA (auth: S), RA (auth: s)
Chain Length:240
Number of Molecules:2
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:Proteasome subunit beta type-
Chain IDs:EA (auth: T), SA (auth: t)
Chain Length:263
Number of Molecules:2
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:26S proteasome non-ATPase reg
Chain IDs:A (auth: U)
Chain Length:921
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:26S proteasome non-ATPase reg
Chain IDs:B (auth: V)
Chain Length:14
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:26S proteasome non-ATPase reg
Chain IDs:C (auth: W)
Chain Length:456
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:26S proteasome non-ATPase reg
Chain IDs:D (auth: X)
Chain Length:422
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:26S proteasome non-ATPase reg
Chain IDs:E (auth: Y)
Chain Length:389
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:26S proteasome non-ATPase reg
Chain IDs:F (auth: Z)
Chain Length:324
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:26S proteasome non-ATPase reg
Chain IDs:G (auth: a)
Chain Length:433
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:26S proteasome non-ATPase reg
Chain IDs:H (auth: b)
Chain Length:377
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:26S proteasome non-ATPase reg
Chain IDs:I (auth: c)
Chain Length:310
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:26S proteasome non-ATPase reg
Chain IDs:J (auth: d)
Chain Length:418
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:26S proteasome complex subuni
Chain IDs:K (auth: e)
Chain Length:389
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:Midnolin
Chain IDs:TA (auth: f)
Chain Length:468
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:substrate peptide
Chain IDs:VA (auth: v)
Chain Length:14
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:26S proteasome non-ATPase reg
Chain IDs:UA (auth: x)
Chain Length:422
Number of Molecules:1
Biological Source:Homo sapiens

Ligand Molecules