ISDA: 9MB7

Deposition Date 2025-03-15

Release Date 2026-03-18

Last Version Date 2026-03-18

Entry Detail

PDB ID:

9MB7

Keywords:

DNA BINDING PROTEIN

Title:

Cryo-EM structure of eSaCas9-NNG-guide RNA-mismatched target DNA complex

Biological Source:

Source Organism(s):

Staphylococcus aureus (Taxon ID: 1280)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

2.88 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

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Macromolecular Entities

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Ubiquitin-like protein SMT3,C
Gene (Uniprot):cas9, SMT3
Chain IDs:C (auth: A)
Chain Length:1170
Number of Molecules:1
Biological Source:Staphylococcus aureus

UniProt ID:Q12306,J7RUA5 Pfam ID:PF00240

Polymer Type:polyribonucleotide
Molecule:sgRNA
Chain IDs:D (auth: B)
Chain Length:99
Number of Molecules:1
Biological Source:Staphylococcus aureus

Polymer Type:polydeoxyribonucleotide
Molecule:target DNA strand
Chain IDs:A (auth: C)
Chain Length:43
Number of Molecules:1
Biological Source:Staphylococcus aureus

Polymer Type:polydeoxyribonucleotide
Molecule:non-target DNA strand
Chain IDs:B (auth: D)
Chain Length:43
Number of Molecules:1
Biological Source:Staphylococcus aureus

Ligand Molecules

Primary Citation

Molecular engineering and structural mechanism of the compact CRISPR-Cas9 nuclease

Nakagawa, R, Omura, S.N, Kajimoto, S, Okazaki, S, Ishiguro, S, Mori, H, Kashiwakura, Y, Hiramoto, T, Hirano, H, Yamashita, K, Jividen, K, Tsai, S.Q, Yachie, N, Ohmori, T, Nishimasu, H, Nureki, O.Show

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Primary Citation of related structures

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