9LZT image
Deposition Date 2025-02-22
Release Date 2025-10-08
Last Version Date 2026-03-11
Entry Detail
PDB ID:
9LZT
Keywords:
RNA BINDING PROTEIN/RNA
Title:
RfxCas13d-crRNA binary complex
Biological Source:
Source Organism(s):
Ruminococcus sp. XPD3002 (Taxon ID: 1452269)
Cloning vector pgRNA-ccdB-1 (Taxon ID: 2760382)
Expression System(s):
Escherichia coli bl21(de3)
Method Details:
Experimental Method:
ELECTRON MICROSCOPY
Resolution:
3.10 Å
Aggregation State:
PARTICLE
Reconstruction Method:
SINGLE PARTICLE
9LZT validation report
Macromolecular Entities
Polymer Type:polyribonucleotide
Molecule:RNA (51-MER)
Chain IDs:A
Chain Length:51
Number of Molecules:1
Biological Source:Cloning vector pgRNA-ccdB-1
Polymer Type:polypeptide(L)
Molecule:RfxCas13d
Chain IDs:B
Chain Length:966
Number of Molecules:1
Biological Source:Ruminococcus sp. XPD3002
Ligand Molecules
MG
Primary Citation
Cryo-EM structure of the RfxCas13d-crRNA-off-target-RNA complex.
Structure 34 11 19.e2 (2026)
PMID: 41135510 DOI: 10.1016/j.str.2025.09.010
Primary Citation of related structures
9LZT
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