ISDA: 9LJ4

Deposition Date 2025-01-14

Release Date 2026-01-21

Last Version Date 2026-04-08

Entry Detail

PDB ID:

9LJ4

Keywords:

DNA BINDING PROTEIN

Title:

CryoEM Structures Uncover the Unexpected Hinges of IscB for Enhanced Gene Editing

Biological Source:

Source Organism(s):

synthetic construct (Taxon ID: 32630)

Expression System(s):

Escherichia coli, Synthetic construct

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

3.09 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

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Macromolecular Entities

Polymer Type:polypeptide(L)
Molecule:Iscb+twin-strep-tag
Chain IDs:A
Chain Length:547
Number of Molecules:1
Biological Source:synthetic construct

Polymer Type:polyribonucleotide
Molecule:RNA (190-MER)
Chain IDs:B
Chain Length:190
Number of Molecules:1
Biological Source:synthetic construct

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (5'-D(P*TP*CP*AP*AP*CP*CP
Chain IDs:C
Chain Length:12
Number of Molecules:1
Biological Source:synthetic construct

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (5'-D(P*TP*AP*GP*GP*TP*GP
Chain IDs:D
Chain Length:17
Number of Molecules:1
Biological Source:synthetic construct

Ligand Molecules

Primary Citation

Structural insight into IscB's RNA-lid-based inactivation mechanism.

Wang, F, Guo, R, Zhang, S, Cui, Y, Wang, J, Hu, T, Liu, K, Wang, Q, Liu, Y, Nam, K.H, Zhao, Z.W, Ji, Q, Xu, X, Wang, E, Zhu, Y, Yang, Y, Luo, M, Ma, P, Ma, S, Xu, C, Hu, C.Show

Nat.Struct.Mol.Biol. ? ? ? (2026)

PMID: 41882346 DOI: 10.1038/s41594-026-01761-3

Primary Citation of related structures

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