ISDA: 9H6T

Deposition Date 2024-10-25

Release Date 2026-02-18

Last Version Date 2026-03-18

Entry Detail

PDB ID:

9H6T

Keywords:

DNA BINDING PROTEIN

Title:

Partial Cas1-Cas2 CRISPR integrase complex bound to a half-integration product, Streptococcus thermophilus DGCC 7710 CRISPR3 system

Biological Source:

Source Organism(s):

Streptococcus thermophilus DGCC 7710 (Taxon ID: 1268061)
synthetic construct (Taxon ID: 32630)

Expression System(s):

Escherichia coli bl21(de3)

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

2.93 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

Download Validation Report

Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:CRISPR-associated endoribonuc
Gene (Uniprot):cas2
Chain IDs:A, D
Chain Length:114
Number of Molecules:2
Biological Source:Streptococcus thermophilus DGCC 7710

UniProt ID:G3ECR3

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:CRISPR-associated endonucleas
Gene (Uniprot):cas1
Chain IDs:B, C
Chain Length:289
Number of Molecules:2
Biological Source:Streptococcus thermophilus DGCC 7710

UniProt ID:G3ECR2

Polymer Type:polydeoxyribonucleotide
Molecule:Fragment of Leader DNA adjace
Chain IDs:E
Chain Length:6
Number of Molecules:1
Biological Source:synthetic construct

Polymer Type:polydeoxyribonucleotide
Molecule:Integration target DNA, the i
Chain IDs:F
Chain Length:48
Number of Molecules:1
Biological Source:synthetic construct

Polymer Type:polydeoxyribonucleotide
Molecule:Integration product
Chain IDs:G
Chain Length:75
Number of Molecules:1
Biological Source:synthetic construct

Polymer Type:polydeoxyribonucleotide
Molecule:DNA prespacer, the non-integr
Chain IDs:H
Chain Length:33
Number of Molecules:1
Biological Source:synthetic construct

Ligand Molecules

Primary Citation

Structural insights into Cas9-mediated prespacer selection in CRISPR-Cas adaptation.

Gaizauskaite, U, Tamulaitiene, G, Silanskas, A, Gasiunas, G, Siksnys, V, Sasnauskas, G.Show

Mol.Cell 86 791 804.e9 (2026)

PMID: 41702403 DOI: 10.1016/j.molcel.2026.01.022

Primary Citation of related structures

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