ISDA: 9H4J

Deposition Date 2024-10-20

Release Date 2026-04-01

Last Version Date 2026-04-08

Entry Detail

PDB ID:

9H4J

Keywords:

HYDROLASE

Title:

dCas9 bound to on-target EMX1-1 (-)SC DNA minicircle

Biological Source:

Source Organism(s):

Streptococcus pyogenes (Taxon ID: 1314)
synthetic construct (Taxon ID: 32630)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

3.10 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

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Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:CRISPR-associated endonucleas
Gene (Uniprot):cas9
Chain IDs:A
Chain Length:1368
Number of Molecules:1
Biological Source:Streptococcus pyogenes

UniProt ID:Q99ZW2 Pfam ID:PF16593, PF16592, PF13395, PF22702, PF16595

Polymer Type:polyribonucleotide
Molecule:EMX1-1 sgRNA
Chain IDs:B
Chain Length:99
Number of Molecules:1
Biological Source:synthetic construct

Polymer Type:polydeoxyribonucleotide
Molecule:Target Strand
Chain IDs:C
Chain Length:45
Number of Molecules:1
Biological Source:synthetic construct

Polymer Type:polydeoxyribonucleotide
Molecule:Non-Target Strand
Chain IDs:D
Chain Length:43
Number of Molecules:1
Biological Source:synthetic construct

Ligand Molecules

Primary Citation

Structural basis of supercoiling-induced CRISPR-Cas9 off-target activity.

Smith, Q.M, Whittle, S, Aramayo, R.J, Rollins, D.E, Jalal, A.S.B, Egharevba, D.I, Morris, K.L, Pyne, A.L.B, Rueda, D.S.Show

Nature ? ? ? (2026)

PMID: 41882360 DOI: 10.1038/s41586-026-10255-7

Primary Citation of related structures

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