ISDA: 8YW3

Deposition Date 2024-03-29

Release Date 2024-09-18

Last Version Date 2024-09-25

Entry Detail

PDB ID:

8YW3

Keywords:

STRUCTURAL PROTEIN

Title:

Cryo-EM structure of the retatrutide-bound human GLP-1R-Gs complex

Biological Source:

Source Organism(s):

synthetic construct (Taxon ID: 32630)
Homo sapiens (Taxon ID: 9606)

Expression System(s):

Escherichia coli, Spodoptera frugiperda

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

2.68 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

Download Validation Report

Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Guanine nucleotide-binding pr
Gene (Uniprot):GNAS
Mutagens:S54N, G226A, E268A, N271K, K274D, R280K, T284D, I285T
Chain IDs:C (auth: A)
Chain Length:394
Number of Molecules:1
Biological Source:Homo sapiens

UniProt ID:P63092 Pfam ID:PF00503

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Guanine nucleotide-binding pr
Gene (Uniprot):GNB1
Chain IDs:B
Chain Length:345
Number of Molecules:1
Biological Source:Homo sapiens

UniProt ID:P62873 Pfam ID:PF00400, PF25391

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Guanine nucleotide-binding pr
Gene (Uniprot):GNG2
Chain IDs:E (auth: G)
Chain Length:71
Number of Molecules:1
Biological Source:Homo sapiens

UniProt ID:P59768 Pfam ID:PF00631

Polymer Type:polypeptide(L)
Molecule:Nanobody-35
Chain IDs:A (auth: N)
Chain Length:140
Number of Molecules:1
Biological Source:synthetic construct

Polymer Type:polypeptide(L)
Molecule:Retatrutide
Chain IDs:F (auth: P)
Chain Length:30
Number of Molecules:1
Biological Source:Homo sapiens

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Glucagon-like peptide 1 recep
Gene (Uniprot):GLP1R
Chain IDs:D (auth: R)
Chain Length:440
Number of Molecules:1
Biological Source:Homo sapiens

UniProt ID:P43220 Pfam ID:PF02793, PF00002

Ligand Molecules

Primary Citation

Structural insights into the triple agonism at GLP-1R, GIPR and GCGR manifested by retatrutide.

Li, W, Zhou, Q, Cong, Z, Yuan, Q, Li, W, Zhao, F, Xu, H.E, Zhao, L.H, Yang, D, Wang, M.W.Show

Cell Discov 10 77 77 (2024)

PMID: 39019866 DOI: 10.1038/s41421-024-00700-0

Primary Citation of related structures

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