8VF2 image
Deposition Date 2023-12-21
Release Date 2025-12-10
Last Version Date 2026-04-08
Entry Detail
PDB ID:
8VF2
Keywords:
DNA BINDING PROTEIN/DNA
Title:
CryoEM structure of Ku homodimer super-complex with linear DNA
Biological Source:
Source Organism(s):
Mycobacterium tuberculosis H37Rv (Taxon ID: 83332)
synthetic construct (Taxon ID: 32360)
Expression System(s):
Escherichia coli bl21(de3)
Method Details:
Experimental Method:
ELECTRON MICROSCOPY
Resolution:
3.70 Å
Aggregation State:
PARTICLE
Reconstruction Method:
SINGLE PARTICLE
8VF2 validation report
Macromolecular Entities
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Non-homologous end joining pr
Gene (Uniprot):mku
Chain IDs:C (auth: A), D (auth: B), E (auth: C), F (auth: D), G (auth: E), H (auth: F)
Chain Length:295
Number of Molecules:6
Biological Source:Mycobacterium tuberculosis H37Rv
UniProt ID:P9WKD9 Pfam ID:PF02735
Polymer Type:polydeoxyribonucleotide
Molecule:DNA (40-MER)
Chain IDs:A (auth: I), I (auth: G)
Chain Length:40
Number of Molecules:2
Biological Source:synthetic construct
Polymer Type:polydeoxyribonucleotide
Molecule:DNA (40-MER)
Chain IDs:B (auth: J), J (auth: H)
Chain Length:40
Number of Molecules:2
Biological Source:synthetic construct
Ligand Molecules
NA
Primary Citation
Bringing the ends together: cryo-EM structures of mycobacterial Ku in complex with DNA define its role in NHEJ synapsis.
Nucleic Acids Res. 54 ? ? (2026)
PMID: 41521670 DOI: 10.1093/nar/gkaf1418
Primary Citation of related structures
8VF2
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