ISDA: 8SUP

Deposition Date 2023-05-12

Release Date 2026-03-18

Last Version Date 2026-04-01

Entry Detail

PDB ID:

8SUP

Keywords:

RIBOSOME

Title:

Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES

Biological Source:

Source Organism(s):

Oryctolagus cuniculus (Taxon ID: 9986)
Homo sapiens (Taxon ID: 9606)
Encephalomyocarditis virus (Taxon ID: 12104)

Expression System(s):

Oryctolagus cuniculus, Homo sapiens

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

3.10 Å

Aggregation State:

CELL

Reconstruction Method:

SINGLE PARTICLE

Download Validation Report

Macromolecular Entities

Polymer Type:polyribonucleotide
Molecule:18S rRNA
Chain IDs:I (auth: 2)
Chain Length:1870
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polyribonucleotide
Molecule:EMCV mRNA
Chain IDs:J (auth: 3)
Chain Length:89
Number of Molecules:1
Biological Source:Encephalomyocarditis virus

Polymer Type:polypeptide(L)
Molecule:Eukaryotic translation initia
Chain IDs:K (auth: A)
Chain Length:124
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:uS2 (SA)
Chain IDs:L (auth: B)
Chain Length:295
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:eS1
Chain IDs:M (auth: C)
Chain Length:84
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:40S ribosomal protein S2
Chain IDs:N (auth: D)
Chain Length:221
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:uS3
Chain IDs:O (auth: E)
Chain Length:281
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:eS4 (S4 X isoform)
Chain IDs:P (auth: F)
Chain Length:133
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:uS7
Chain IDs:Q (auth: G)
Chain Length:204
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:eS6
Chain IDs:R (auth: H)
Chain Length:317
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:eS7
Chain IDs:S (auth: I)
Chain Length:432
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:eS8
Chain IDs:T (auth: J)
Chain Length:315
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:uS4
Chain IDs:U (auth: K)
Chain Length:194
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:eS10
Chain IDs:V (auth: L)
Chain Length:149
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:uS17
Chain IDs:W (auth: M)
Chain Length:158
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:eS12
Chain IDs:X (auth: N)
Chain Length:132
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:uS15
Chain IDs:Y (auth: O)
Chain Length:1362
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:Small ribosomal subunit prote
Chain IDs:Z (auth: P)
Chain Length:843
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:uS19
Chain IDs:AA (auth: Q)
Chain Length:445
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:uS9
Chain IDs:BA (auth: R)
Chain Length:172
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:eS17
Chain IDs:CA (auth: S)
Chain Length:135
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:uS13
Chain IDs:DA (auth: T)
Chain Length:218
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:eS19
Chain IDs:EA (auth: U)
Chain Length:145
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:uS10
Chain IDs:FA (auth: V)
Chain Length:374
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:eS21
Chain IDs:GA (auth: W)
Chain Length:83
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:uS8
Chain IDs:HA (auth: X)
Chain Length:130
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:uS12
Chain IDs:IA (auth: Y)
Chain Length:143
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:eS24
Chain IDs:JA (auth: Z)
Chain Length:131
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:eS25
Chain IDs:KA (auth: a)
Chain Length:124
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:eS26
Chain IDs:LA (auth: b)
Chain Length:295
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:eS27
Chain IDs:MA (auth: c)
Chain Length:84
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:eS28
Chain IDs:NA (auth: d)
Chain Length:221
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:uS14
Chain IDs:OA (auth: e)
Chain Length:281
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:eS30
Chain IDs:PA (auth: f)
Chain Length:133
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:eS31
Chain IDs:QA (auth: g)
Chain Length:204
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:Receptor for Activated C Kina
Chain IDs:RA (auth: h)
Chain Length:317
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polyribonucleotide
Molecule:Met-tRNAiMet
Chain IDs:SA (auth: i)
Chain Length:432
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:Eukaryotic translation initia
Chain IDs:TA (auth: j)
Chain Length:315
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:eL41
Chain IDs:UA (auth: n)
Chain Length:132
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:Eukaryotic translation initia
Chain IDs:A (auth: o)
Chain Length:1362
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:Eukaryotic translation initia
Chain IDs:B (auth: p)
Chain Length:843
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:Eukaryotic translation initia
Chain IDs:C (auth: q)
Chain Length:445
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:Eukaryotic translation initia
Chain IDs:D (auth: r)
Chain Length:172
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:Eukaryotic translation initia
Chain IDs:E (auth: s)
Chain Length:135
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:Eukaryotic translation initia
Chain IDs:F (auth: t)
Chain Length:218
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:Eukaryotic translation initia
Chain IDs:G (auth: u)
Chain Length:145
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Polymer Type:polypeptide(L)
Molecule:Eukaryotic translation initia
Chain IDs:H (auth: v)
Chain Length:374
Number of Molecules:1
Biological Source:Oryctolagus cuniculus

Ligand Molecules

Primary Citation

The mechanism of ribosomal recruitment during translation initiation on the Type 2 encephalomyocarditis virus IRES.

Bhattacharjee, S, Abaeva, I.S, Brown, Z.P, Arhab, Y, Fallah, H, Hellen, C.U.T, Frank, J, Pestova, T.V, Bhattacharjee, S, Abaeva, I.S, Brown, Z.P, Arhab, Y, Fallah, H, Hellen, C.U.T, Frank, J, Pestova, T.V.Show

Embo J. ? ? ? (2026)

PMID: 41851500 DOI: 10.1038/s44318-026-00735-x

Primary Citation of related structures

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