ISDA: 8PJ9

Deposition Date 2023-06-23

Release Date 2024-07-10

Last Version Date 2026-03-04

Entry Detail

PDB ID:

8PJ9

Keywords:

RNA BINDING PROTEIN

Title:

Cas9 bound to cognate DNA, Streptococcus thermophilus DGCC 7710 CRISPR3 system

Biological Source:

Source Organism(s):

Streptococcus thermophilus DGCC 7710 (Taxon ID: 1268061)
synthetic construct (Taxon ID: 32630)

Expression System(s):

Escherichia coli str. k-12 substr. dh10b

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

3.24 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

Download Validation Report

Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:CRISPR-associated endonucleas
Gene (Uniprot):cas9
Chain IDs:A
Chain Length:1397
Number of Molecules:1
Biological Source:Streptococcus thermophilus DGCC 7710

UniProt ID:G3ECR1 Pfam ID:PF16593, PF16592, PF13395, PF22702, PF16595

Polymer Type:polyribonucleotide
Molecule:crRNA, chain B
Chain IDs:B
Chain Length:42
Number of Molecules:1
Biological Source:Streptococcus thermophilus DGCC 7710

Polymer Type:polyribonucleotide
Molecule:tracrRNA, chain C
Chain IDs:C
Chain Length:75
Number of Molecules:1
Biological Source:Streptococcus thermophilus DGCC 7710

Polymer Type:polydeoxyribonucleotide
Molecule:DNA oligoduplex, target stran
Chain IDs:D, E
Chain Length:32
Number of Molecules:2
Biological Source:synthetic construct

Polymer Type:polydeoxyribonucleotide
Molecule:DNA oligoduplex, non-target s
Chain IDs:F
Chain Length:12
Number of Molecules:1
Biological Source:synthetic construct

Ligand Molecules

Primary Citation

Structural insights into Cas9-mediated prespacer selection in CRISPR-Cas adaptation.

Gaizauskaite, U, Tamulaitiene, G, Silanskas, A, Gasiunas, G, Siksnys, V, Sasnauskas, G.Show

Mol.Cell ? ? ? (2026)

PMID: 41702403 DOI: 10.1016/j.molcel.2026.01.022

Primary Citation of related structures

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