ISDA: 24PI

Deposition Date 2026-03-15

Release Date 2026-03-25

Last Version Date 2026-03-25

Entry Detail

PDB ID:

24PI

Keywords:

TRANSFERASE

Title:

Ancestrally reconstructed acetolactate synthase - Ancestor N1774 with bound ThDP and magnesium.

Biological Source:

Source Organism(s):

synthetic construct (Taxon ID: 32630)

Expression System(s):

Escherichia coli bl21(de3)

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

2.75 Å

R-Value Free:

0.23

R-Value Work:

0.20

R-Value Observed:

0.20

Space Group:

P 1 21 1

Download Validation Report

Macromolecular Entities

Polymer Type:polypeptide(L)
Molecule:Ancestral acetolactate syntha
Chain IDs:A, B, C, D
Chain Length:569
Number of Molecules:4
Biological Source:synthetic construct

Ligand Molecules

ACT

GOL

TPP

Primary Citation

Engineering of Acetolactate Synthase for Isobutanol Production Using Ancestral Sequence Reconstruction

Douw, A, Krco, S, Bayaraa, T, Foley, G, Davis, S.J, Paredes Trujillo, O, Asser, L, Sieber, V, Boden, M, Hine, D, Guddat, L.W, Schenk, G.Show

To Be Published ? ? ? (?)

Primary Citation of related structures

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