ELECTRON MICROSCOPY

Sample

Saccharomyces cerevisiae outer kinetochore KMN junction complex

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK III
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 113688
Reported Resolution (Å) 4.9
Resolution Method FSC 0.143 CUT-OFF
Other Details Composite cryo-EM of two multibody refinement-derived map refined to the consensus map.
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol FLEXIBLE FIT
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details Flexible fitting using Isolde, real-space refinement using Phenix
Data Acquisition
Detector Type GATAN K3 (6k x 4k)
Electron Dose (electrons/Å2) 40
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 1400
Maximum Defocus (nm) 3000
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS ?
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification 105000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION Topaz ?
MODEL REFINEMENT PHENIX 1.21.2_5419:
CTF CORRECTION CTFFIND 4.1
INITIAL EULER ASSIGNMENT RELION 5
FINAL EULER ASSIGNMENT RELION 5
CLASSIFICATION RELION 5
RECONSTRUCTION RELION 5
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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