ELECTRON MICROSCOPY

Sample

CorM filament in the presence of CorR from cyanobacterium Anabaena sp. PCC 7120

Specimen Preperation
Sample Aggregation State FILAMENT
Vitrification Instrument LEICA EM GP
Cryogen Name ETHANE
Sample Vitrification Details Vitrification was carried out after 3-3.5s of blotting
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 124109
Reported Resolution (Å) 3.92
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol FLEXIBLE FIT
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type FEI FALCON IV (4k x 4k)
Electron Dose (electrons/Å2) 40
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS GLACIOS
Minimum Defocus (nm) 900
Maximum Defocus (nm) 3500
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification 150000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 200
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC 4.4
IMAGE ACQUISITION EPU 3.7.0
CTF CORRECTION cryoSPARC 4.4
MODEL FITTING UCSF ChimeraX 1.9
INITIAL EULER ASSIGNMENT cryoSPARC 4.4
FINAL EULER ASSIGNMENT cryoSPARC 4.4
CLASSIFICATION cryoSPARC 4.4
RECONSTRUCTION cryoSPARC 4.4
MODEL REFINEMENT ISOLDE v.19
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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