| X-RAY DIFFRACTION |
8.0 |
293 |
Reservoir solution: PEG3350 (9% w/v), HEPES (50mM; pH 8.0)
Protein solution: mandelate racemase (10 mg/ml) + 500uM Tavaborole in 100mM HEPES, pH 7.5, 3.3mM MgCl2
2 uL of protein solution was mixed with 2 uL of well solution.
Cube-like crystals grew spontaneously over 18-25 days.
Crystals were equilibrated in synthetic stabilizing solution (10% w/v PEG3350, 50 mM HEPES, pH 8.0, 5 mM MgCl2, 500 uM Tavaborole, 5% v/v ethylene glycol) for 5 minutes and then transferred directly to cryoprotectant solution (10% w/v PEG3350, 50 mM HEPES, pH 8.0, 5 mM MgCl2, 500 uM Tavaborole, 20% v/v ethylene glycol) for 5 minutes prior to flash freezing in liquid nitrogen. |
