| X-RAY DIFFRACTION |
5.5 |
291 |
In a 2 mL Eppendorf Tube, 0.5 mL of precipitant (0.4M NaOAc pH=5.5, 16% PEG-3350) and 0.5 mL of SLO (10 mg/mL in 50 mM NaOAc, pH=5.0) was combined together with ~10uL of seed stock (see 2.2.1.) being added to the tube cap prior to mixing by inversion. The tube was then placed on a Thermo Fisher Tuber Revolver Rotator, set to 30 RPM. Samples were left to mix overnight. Subsequently, samples were mixed in a ~1:1 ratio with 18% hydroxyethylcellulose (Sigma-Aldrich PN-09368) dissolved in SLO precipitant (0.4M NaOAc pH=5.5, 16% PEG-3350) |
