ELECTRON MICROSCOPY

Sample

Reconstituted yeast V-ATPase bound to Rtc5p

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument LEICA EM GP
Cryogen Name ETHANE
Sample Vitrification Details Leica EM GP2
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 90977
Reported Resolution (Å) 3.6
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space ?
Refinement Protocol ?
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type TFS FALCON 4i (4k x 4k)
Electron Dose (electrons/Å2) 52.5
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS GLACIOS
Minimum Defocus (nm) 400
Maximum Defocus (nm) 2000
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model ?
Nominal Magnification 92000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 200
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION Topaz ?
CTF CORRECTION CTFFIND 4.1.14
MODEL FITTING Coot ?
MODEL FITTING ISOLDE ?
INITIAL EULER ASSIGNMENT RELION 5
FINAL EULER ASSIGNMENT RELION 5
RECONSTRUCTION RELION 5
MODEL REFINEMENT PHENIX 1.21_5207
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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