ELECTRON MICROSCOPY

Sample

Polyrod composed of FlgG(G65V)

Specimen Preperation
Sample Aggregation State FILAMENT
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name NITROGEN
Sample Vitrification Details blotting time of 4 seconds, 1 seconds drain time, force 0
3D Reconstruction
Reconstruction Method HELICAL
Number of Particles 88969
Reported Resolution (Å) 3.37
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type HELICAL
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol OTHER
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details Phenix1.21-rc1 , refmac servalcat and ISOLDE was used for fitting, and Phenix was used for final fitting.
Data Acquisition
Detector Type GATAN K3 (6k x 4k)
Electron Dose (electrons/Å2) 75
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model JEOL CRYO ARM 300
Minimum Defocus (nm) 200
Maximum Defocus (nm) 2000
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS ?
Imaging Mode BRIGHT FIELD
Specimen Holder Model ?
Nominal Magnification 50000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC 3.2.0
MODEL REFINEMENT PHENIX 1.21rc1_5127
CTF CORRECTION cryoSPARC 3.2.0
CLASSIFICATION cryoSPARC 3.2.0
RECONSTRUCTION cryoSPARC 3.2.0
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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