ELECTRON MICROSCOPY

Sample

GTP cyclohydrolase 1

Specimen Preperation
Sample Aggregation State 2D ARRAY
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 565880
Reported Resolution (Å) 2.77
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type 3D CRYSTAL
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol FLEXIBLE FIT
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type FEI FALCON IV (4k x 4k)
Electron Dose (electrons/Å2) 60
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS GLACIOS
Minimum Defocus (nm) 200
Maximum Defocus (nm) 600
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS ?
Imaging Mode BRIGHT FIELD
Specimen Holder Model ?
Nominal Magnification 100000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 200
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC 5.0
CTF CORRECTION cryoSPARC 5.0
MODEL FITTING UCSF ChimeraX 1.11.1
MODEL REFINEMENT PHENIX 2.0_5936
INITIAL EULER ASSIGNMENT cryoSPARC 5.0
CLASSIFICATION cryoSPARC 5.0
RECONSTRUCTION cryoSPARC 5.0
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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