Raw file Experiment Fraction Enzyme Enzyme mode Enzyme first search Enzyme mode first search Use enzyme first search Variable modifications Fixed modifications Multi modifications Variable modifications first search Use variable modifications first search Requantify Multiplicity Max. missed cleavages Max. labeled AAs Labels0 Labels1 Labels2 LC-MS run type Time-dependent recalibration MS MS/MS MS3 MS/MS Submitted MS/MS Submitted (SIL) MS/MS Submitted (ISO) MS/MS Submitted (PEAK) MS/MS Identified MS/MS Identified (SIL) MS/MS Identified (ISO) MS/MS Identified (PEAK) MS/MS Identified [%] MS/MS Identified (SIL) [%] MS/MS Identified (ISO) [%] MS/MS Identified (PEAK) [%] Peptide Sequences Identified Peaks Peaks Sequenced Peaks Sequenced [%] Peaks Repeatedly Sequenced Peaks Repeatedly Sequenced [%] Isotope Patterns Isotope Patterns Sequenced Isotope Patterns Sequenced (z>1) Isotope Patterns Sequenced [%] Isotope Patterns Sequenced (z>1) [%] Isotope Patterns Repeatedly Sequenced Isotope Patterns Repeatedly Sequenced [%] Multiplets Multiplets z=1 Multiplets z=2 Multiplets z=3 Multiplets z=4 Multiplets z=5 Multiplets z=6 Multiplets z=7 Multiplets Sequenced Multiplets Sequenced [%] Multiplets Repeatedly Sequenced Multiplets Repeatedly Sequenced [%] Multiplets Identified Multiplets Identified [%] Recalibrated Av. Absolute Mass Deviation [ppm] Mass Standard Deviation [ppm] Av. Absolute Mass Deviation [mDa] Mass Standard Deviation [mDa] ESC-HF-S1-T-1-12-02-20-Shemin-18-2-20 Exp 2 1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 5877 20001 0 24560 8130 7312 9118 9168 6355 2561 252 37 78 35 2.8 4488 516881 17438 3.4 837 4.8 58764 14251 13841 24 27 994 7 5230 98 2989 1838 288 17 0 0 3957 76 2805 71 3160 80 + 0.54526 0.73889 0.36438 0.5247 ESC-HF-S2-T-1-28-01-20-Shemin Exp 1 1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 6090 21087 0 23738 6729 11707 5302 8168 5127 2780 261 34 76 24 4.9 4263 860617 18940 2.2 1212 6.4 96829 16367 15807 17 21 1491 9.1 4639 106 2812 1459 194 35 20 13 3379 73 2294 68 2640 78 + 0.5756 0.77239 0.37002 0.51704 ESC-HF-S2-T-2-12-02-20-Shemin-18-2-20 Exp 2 2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 5959 20516 0 24003 8889 8140 6974 10908 7231 3332 345 45 81 41 4.9 5722 675742 18452 2.7 690 3.7 82627 15996 15627 19 22 917 5.7 6309 105 3493 2299 365 39 7 1 4556 72 3079 68 3788 83 + 0.6006 0.80386 0.40007 0.56313 ESC-HF-S3-T-2-28-01-20-Shemin Exp 1 2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 5702 22930 0 25692 8992 11176 5524 10605 6986 3242 377 41 78 29 6.8 5329 763644 20868 2.7 1207 5.8 94607 18130 17584 19 22 1502 8.3 5979 147 3366 2073 306 39 28 20 4299 72 3010 70 3421 80 + 0.59549 0.79701 0.39042 0.55159 ESC-HF-S3-T-3-12-02-20-Shemin-18-2-20-rep Exp 2 3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 7013 16707 0 20897 6695 5822 8380 6951 5013 1767 171 33 75 30 2 3611 676846 14465 2.1 552 3.8 61262 11754 11450 19 23 676 5.8 4282 66 2663 1348 184 18 1 2 3248 76 2322 71 2506 77 + 0.54434 0.73632 0.35775 0.51237 ESC-HF-S4-T-3-28-01-20-Shemin Exp 1 3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 6092 21308 0 23997 8198 10421 5378 8790 5843 2594 353 37 71 25 6.6 4558 714434 19099 2.7 1354 7.1 84805 16420 15839 19 22 1550 9.4 5330 203 3264 1586 210 37 22 8 3807 71 2662 70 2823 74 + 0.5998 0.80164 0.36653 0.50674 ESC-HF-S4-T-4-12-02-20-Shemin-18-2-20 Exp 2 4 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 4872 22697 0 27787 9276 8331 10180 10351 7163 2882 306 37 77 35 3 4989 500053 19628 3.9 937 4.8 71037 16244 15787 23 25 1145 7 6308 167 3514 2224 337 56 9 1 4500 71 3136 70 3482 77 + 0.56157 0.74172 0.38255 0.5351 ESC-HF-S5-T-4-28-01-20-Shemin Exp 1 4 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 5973 22233 0 24859 9110 10497 5252 8488 5980 2244 264 34 66 21 5 3868 748218 19924 2.7 1412 7.1 89315 17323 16752 19 23 1669 9.6 5999 415 3531 1713 278 47 12 3 4100 68 2914 71 2712 66 + 0.5814 0.76818 0.3681 0.50949 ESC-HF-S5-T-5-12-02-20-Shemin-18-2-20 Exp 2 5 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 5173 23013 0 28232 9359 8435 10438 10985 7302 3326 357 39 78 39 3.4 5273 667744 19643 2.9 972 4.9 88014 16250 15822 18 20 1264 7.8 6178 240 3352 2096 368 55 40 27 4422 72 3073 69 3499 79 + 0.57873 0.76892 0.39391 0.55926 ESC-HF-S6-T-5-28-01-20-Shemin Exp 1 5 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 5475 23465 0 26315 9538 11077 5700 10267 6772 3132 363 39 71 28 6.4 4704 659922 21186 3.2 1382 6.5 86298 18235 17665 21 24 1739 9.5 5944 282 3349 1939 286 45 36 7 4322 73 3040 70 3124 72 + 0.5751 0.76261 0.3758 0.52398 ESC-HF-S6-T-6-12-02-20-Shemin-18-2-20 Exp 2 6 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 4338 24914 0 30351 9668 9809 10874 10460 7071 3104 285 34 73 32 2.6 4760 631760 21215 3.4 1359 6.4 88510 17281 16751 20 21 1724 10 6193 266 3427 2049 323 50 50 28 4426 71 3127 71 3264 74 + 0.57625 0.77021 0.39345 0.55901 ESC-HF-S7-T-6-28-01-20-Shemin Exp 1 6 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 5640 22734 0 25165 9290 11013 4862 8402 5875 2322 205 33 63 21 4.2 3630 716313 20555 2.9 1402 6.8 87774 17937 17425 20 23 1785 10 5803 307 3197 1849 312 72 51 15 4142 71 2928 71 2648 64 + 0.56235 0.7538 0.37365 0.52874 ESC-HF-S7-T-7-12-02-20-Shemin-18-2-20 Exp 2 7 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 4762 22957 0 27939 8758 9217 9964 9400 6374 2718 308 34 73 29 3.1 4059 540255 19637 3.6 1390 7.1 69968 15893 15272 23 25 1665 10 5318 165 2781 1900 379 74 12 7 3968 75 2770 70 2903 73 + 0.55918 0.75418 0.38216 0.54499 ESC-HF-S8-T-7-28-01-20-Shemin Exp 1 7 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 5463 23592 0 26886 9225 11073 6588 7966 5454 2242 270 30 59 20 4.1 3502 614015 20952 3.4 1537 7.3 81242 17824 17156 22 25 1755 9.8 5977 562 3445 1639 259 52 18 2 4110 69 2876 70 2408 59 + 0.59114 0.78292 0.37571 0.51876 ESC-HF-S8-T-8-12-02-20-Shemin-18-2-20 Exp 2 8 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 4532 24398 0 29327 10014 9455 9858 10818 7518 2981 319 37 75 32 3.2 4426 513392 20741 4 1532 7.4 71541 17013 16468 24 26 1917 11 6090 339 3178 2088 389 77 14 5 4422 73 3180 72 3269 74 + 0.55655 0.73973 0.38808 0.55577 ESC-HF-S9-T-8-28-01-20-Shemin Exp 1 8 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 5434 23831 0 26779 10112 10771 5896 8404 5949 2210 245 31 59 21 4.2 3406 667677 21095 3.2 1708 8.1 89118 18059 17512 20 23 2013 11 6435 576 3595 1791 323 80 56 14 4408 69 3154 72 2553 58 + 0.55009 0.73567 0.35398 0.49779 ESC-HF-S9-T-9-12-02-20-Shemin-18-2-20 Exp 2 9 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 5312 21981 0 27445 8889 7628 10928 8194 5693 2235 266 30 64 29 2.4 3479 466242 18301 3.9 1283 7 59089 14552 14104 25 27 1503 10 5299 213 2848 1829 333 70 5 1 3963 75 2828 71 2526 64 + 0.55983 0.73651 0.37758 0.52567 ESC-HF-S10-T-9-28-01-20-Shemin Exp 1 9 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 5801 21883 0 24336 9170 10260 4906 6189 4425 1617 147 25 48 16 3 2478 746475 19046 2.6 1710 9 92371 16471 15968 18 20 2056 12 5739 397 3017 1744 330 92 118 41 3931 68 2830 72 1886 48 + 0.55371 0.72466 0.35983 0.49626 ESC-HF-S10-T-10-12-02-20-Shemin-18-2-20 Exp 2 10 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 5207 22254 0 26937 9267 8304 9366 8807 6201 2348 258 33 67 28 2.8 3672 489900 18837 3.8 1432 7.6 63441 15295 14736 24 27 1664 11 5583 285 2984 1903 340 65 6 0 4151 74 2908 70 2729 66 + 0.58366 0.78104 0.39653 0.56979 ESC-HF-S11-T-10-28-01-20-Shemin Exp 1 10 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 6282 20787 0 23048 8620 9906 4522 5688 4142 1416 130 25 48 14 2.9 2327 703122 18407 2.6 1444 7.8 81479 16163 15548 20 23 1689 10 5690 570 3212 1583 270 44 11 0 3824 67 2671 70 1819 48 + 0.55714 0.74697 0.35154 0.49264 ESC-HF-S11-T-11-12-02-20-Shemin-18-2-20 Exp 2 11 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 5468 21520 0 26278 9248 7514 9516 8695 6098 2347 250 33 66 31 2.6 3808 483769 18337 3.8 1128 6.2 62891 15067 14648 24 27 1334 8.9 5740 225 3115 2007 333 55 5 0 4260 74 3010 71 2782 65 + 0.57368 0.76638 0.38575 0.55089 ESC-HF-S12-T-11-28-01-20-Shemin Exp 1 11 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 5603 21751 0 24156 9026 10320 4810 6753 4673 1880 200 28 52 18 4.2 2818 655337 19637 3 1342 6.8 79308 17096 16560 22 24 1728 10 5757 291 2838 2038 421 110 43 16 4083 71 2860 70 2116 52 + 0.58642 0.76925 0.39461 0.55091 ESC-HF-S12-T-12-12-02-20-Shemin-18-2-20 Exp 2 12 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 5336 21926 0 26667 9582 7601 9484 8593 6041 2336 216 32 63 31 2.3 3562 464568 18565 4 1272 6.9 61024 15259 14842 25 28 1485 9.7 5762 284 3169 1920 318 61 9 1 4247 74 3082 73 2661 63 + 0.56818 0.76238 0.37875 0.53647 ESC-HF-S13-T-12-28-01-20-Shemin Exp 1 12 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 5535 22612 0 25008 9586 10630 4792 6668 4930 1620 118 27 51 15 2.5 2685 685840 19982 2.9 1693 8.5 87220 17445 16886 20 23 2019 12 6173 463 3221 1949 365 95 62 18 4217 68 2999 71 2155 51 + 0.56291 0.73885 0.3706 0.51147 ESC-HF-S13-T-13-12-02-20-Shemin-18-2-20 Exp 2 13 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 5793 21332 0 25549 8931 8184 8434 8562 6107 2213 242 34 68 27 2.9 3586 479383 18188 3.8 1316 7.2 61779 15182 14709 25 28 1492 9.8 5507 427 3277 1572 194 32 5 0 3998 73 2877 72 2682 67 + 0.54805 0.73408 0.36194 0.51004 ESC-HF-S14-T-13-28-01-20-Shemin Exp 1 13 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 5514 22275 0 24895 8863 10792 5240 7620 5442 1965 213 31 61 18 4.1 3105 606707 19930 3.3 1372 6.9 75796 17286 16708 23 26 1707 9.9 5582 355 2976 1814 336 72 24 5 3991 71 2873 72 2418 61 + 0.54998 0.72781 0.36564 0.51338 ESC-HF-S14-T-14-12-02-20-Shemin-18-2-20 Exp 2 14 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 5647 21722 0 26009 9697 7738 8574 8871 6268 2321 282 34 65 30 3.3 3633 472958 18396 3.9 1349 7.3 62170 15371 14929 25 28 1598 10 5857 454 3486 1661 212 38 6 0 4248 73 3029 71 2704 64 + 0.55175 0.72856 0.36037 0.5015 ESC-HF-S15-T-14-28-01-20-Shemin Exp 1 14 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 5569 21828 0 24204 8902 10550 4752 7053 5096 1786 171 29 57 17 3.6 2848 641341 19729 3.1 1409 7.1 77604 17183 16621 22 25 1796 10 5506 384 3008 1709 299 79 24 3 3943 72 2865 73 2212 56 + 0.55265 0.74149 0.36635 0.51538 ESC-HF-S15-T-15-12-02-20-Shemin-18-2-20 Exp 2 15 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 5589 21442 0 25773 8987 8124 8662 8030 5551 2220 259 31 62 27 3 3446 465964 18319 3.9 1332 7.3 60101 15130 14632 25 29 1522 10 5488 383 3252 1570 254 25 4 0 4010 73 2888 72 2464 61 + 0.53019 0.70735 0.34356 0.48408 ESC-HF-S16-T-15-28-01-20-Shemin Exp 1 15 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 5596 23194 0 26009 9918 10461 5630 7148 5297 1701 150 27 53 16 2.7 2882 628108 20526 3.3 1614 7.9 84503 17761 16967 21 25 1946 11 6390 796 3697 1591 229 48 24 5 4340 68 3104 72 2262 52 + 0.54673 0.7235 0.34964 0.48889 ESC-HF-S16-T-16-12-02-20-Shemin-18-2-20 Exp 2 16 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 5762 21357 0 25574 9222 7918 8434 7228 5163 1822 243 28 56 23 2.9 2847 456994 17773 3.9 1540 8.7 56778 14753 14195 26 30 1760 12 5155 495 3096 1393 149 19 3 0 3767 73 2821 75 2073 55 + 0.56219 0.74639 0.37404 0.53555 ESC-HF-S17-T-16-28-01-20-Shemin Exp 1 16 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 5399 22867 0 25837 9027 10870 5940 5779 4173 1400 206 22 46 13 3.5 2143 620541 19847 3.2 1782 9 80266 16882 16211 21 24 2109 12 5191 544 2889 1392 242 70 39 15 3655 70 2667 73 1681 46 + 0.593 0.77531 0.38962 0.53798 ESC-HF-S17-T-17-12-02-20-Shemin-18-2-20 Exp 2 17 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 6436 19683 0 22867 6681 9818 6368 5217 3332 1664 221 23 50 17 3.5 2132 605072 16962 2.8 1489 8.8 60501 14056 13331 23 28 1749 12 3785 434 2393 883 63 10 2 0 2740 72 2027 74 1406 51 + 0.5783 0.77037 0.37123 0.52592 ESC-HF-S18-T-17-28-01-20-Shemin Exp 1 17 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 5160 23696 0 27352 7110 12930 7312 4023 2792 1087 144 15 39 8.4 2 1610 588846 20037 3.4 2060 10 78965 16681 15685 21 25 2368 14 4398 774 2478 936 106 48 46 10 2915 66 2054 70 1154 40 + 0.60915 0.79723 0.38795 0.53698 ESC-HF-S18-T-18-12-02-20-Shemin-18-2-20 Exp 2 18 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 7417 17211 0 21302 3662 9458 8182 2802 1708 992 102 13 47 10 1.2 1257 672345 14319 2.1 1384 9.7 53365 10893 10007 20 27 1525 14 2237 429 1483 316 9 0 0 0 1539 69 1066 69 697 45 + 0.64382 0.82749 0.37702 0.49926 ESC-HF-S19-T-18-28-01-20-Shemin Exp 1 18 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False True 3 2 3 Arg6;Lys4 Arg10;Lys8 Standard 6569 18915 0 22638 3329 11863 7446 2486 1592 805 89 11 48 6.8 1.2 1117 590470 15467 2.6 1681 11 58805 12318 11367 21 27 1887 15 2113 429 1338 312 27 6 1 0 1434 68 971 68 669 47 + 0.63241 0.82969 0.37306 0.50598 Total 203390 786619 0 918411 309700 345125 263586 284537 196737 79212 8588 31 64 23 3.3 46662 21991495 2699167 575821 555412 21 24 58743 10 194966 12676 109733 60013 9631 1832 813 268 139322 71 98800 71 89296 64 0.56936 0.75833 0.37529 0.5285