Study Info
INDA Accessions: INRP000691
INSDC Accessions: PRJEB115936, ERP196082
- Title: Kill Switches
- Data Type : Other
- Descriptive Title: Integrative Omics Approaches to Decipher KILL SWITCHES in Pan Drug Resistant Pathogens isolated from Tunneled Cuffed Catheter Tips
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Other Info
- Abstract: Microbial biofilms pose a significant healthcare scientific societal and economic burden They are associated with various types of infections that can severely impact patients Biofilmassociated infections are often chronic and highly resistant to antibiotic therapy due to intrinsic factors such as extracellular matrix production reduced growth rates altered antibiotic target expression and efficient exchange of resistance genes Hence biofilms are considered as a major contributor to the growing problem of antimicrobial resistance Biofilms formed by critical and highpriority pathogens such as Pseudomonas aeruginosa Klebsiella pneumoniae and Acinetobacter baumannii represent a significant challenge to public health due to their inherent antimicrobial resistance AMR and persistence in diverse environments The proposed research employs an integrative omics framework genomics transcriptomics proteomics and metabolomics to elucidate biofilm formation dynamics identify molecular determinants of AMR and uncover pathways that could serve as potential therapeutic targets By applying stateoftheart sequencing technologies systems biology approaches and advanced computational analyses this project aims to generate a comprehensive multiomics profile of biofilmassociated resistance The findings will inform the development of novel strategies to disrupt biofilm formation and combat AMR addressing a critical need for effective treatment options against priority pathogens Antimicrobial resistance is a global crisis and biofilms exacerbate this problem by creating physical and physiological barriers to antimicrobial agents Biofilms protect bacterial populations and facilitate resistance through horizontal gene transfer altered metabolism and matrix production Critical and high priority pathogens as identified by the WHO and CDC are notorious for biofilmmediated AMR and include carbapenemresistant Pseudomonas aeruginosa Klebsiella pneumoniae and Acinetobacter baumannii These bacteria exhibit several critical biological characteristics including adaptations for survival in contemporary healthcare environments diverse mechanisms for acquiring resistance determinants and the global dissemination of highrisk clonal lineages The advent of nextgeneration sequencing has facilitated the development of advanced tools to monitor and mitigate the spread of these pathogens alongside a renewed focus on innovative nontraditional antibiotic strategies Omics technologies offer unparalleled opportunities to dissect the molecular mechanisms underlying biofilm formation and AMR By integrating genomics transcriptomics proteomics and metabolomics data this project will uncover key regulatory networks and pathways that contribute to biofilm resilience This systemslevel understanding is essential for identifying novel therapeutic targets and strategies to counter biofilmmediated infections At the end project the data generated will be useful to identify the kill switches and small molecules which can transport through biofilm in the nosocomial pathogens from central venous catheter inserted patients which are biofilm formers and pan drug resistant The structure of biofilm matrix will allow us to study the mobility of molecules and trigger the kill switches which can trigger bacterial suicide as a therapeutic approach in the pathogens
- Linked publications:
- Center Name: Dr Dhara N Patel, Bapubhai Desaibhai Patel Institute of Allied and Healthcare Sciences - CHARUSAT; Dr Sishir Gang, Muljibhai Patel Society for Research in Nephro-Urology; Vijay L Odedara, Bapubhai Desaibhai Patel Institute of Allied and Healthcare Sciences - CHARUSAT; Mansi Joshi, Bapubhai Desaibhai Patel Institute of Allied and Healthcare Sciences - CHARUSAT
- Number of Base(Total) Mbp: 0
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