Study Info
INDA Accessions: INRP000679
INSDC Accessions: PRJEB114344, ERP194651
- Title: Genetic engineering of Vibrio cholerae genome to develop a rapid-growth microbial chassis
- Data Type : Whole Genome Sequencing
- Descriptive Title: Engineering Vibrio cholerae BB1 as a Rapid-Growth Chassis for T7 RNA Polymerase expression Driven Recombinant Protein Production
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Organism:
Scientific Name(Taxon Id): Vibrio cholerae O1 biovar El Tor str. N16961 (243277) Common Name:
Other Info
- Abstract: in the present study Vibrio cholerae genome has been genetically modified to simultaneous delete four genomic islands and the TLCCTX region, without introducing any antibiotic resistance markers. These deletions rendered the strain completely avirulent. Further, heterologous expression of the Escherichia coli BL21 DE3- encoded T7 RNA polymerase in this non-toxigenic Vibrio cholerae strain BBI have conferred the strain the capacity to drive T7 promoter dependent gene expression. This enables the development of an alternative Gram-negative chassis which has a shorter doubling time. The system provides a novel controllable expression platform capable of producing higher yields of recombinant proteins.
- Linked publications:
- Center Name: Bhabatosh Das and Centre for MIcrobial Research, THSTI
- Number of Base(Total) Mbp: 0
- Size in bytes(Total):
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- Number of Sequences:
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- Number of Variant files:
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