Study Info
INDA Accessions: INRP000478
INSDC Accessions: PRJEB100756, ERP182202
- Title: RNA Seq Analysis of WT, ospho1-2 and EXS lines under Pi sufficient conditions
- Data Type : RNASeq
- Descriptive Title: RNA Seq Analysis of WT, ospho1-2 and EXS lines under Pi sufficient conditions
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Organism:
Scientific Name(Taxon Id): Oryza sativa (4530) Common Name: Asian cultivated rice
Other Info
- Abstract: The ospho1-2 knock-out and rice lines expressing only the EXS domain of OsPHO1-2 were generated by employing CRISPR-Cas9 technology. Five-day-old seedlings of WT, ospho1-2, and EXS-1 lines were transferred to Pi-sufficient media and grown for 14 days. Total RNA was extracted from the shoot tissues using the Direct-zol RNA kit and quantified with NanoDrop One. Samples with an RNA integrity number above 7.0 were used for library preparation using the KAPA RNA HyperPrep Kit. Three biological replicates for WT, ospho1-2, and EXS-1 lines were sequenced on the Illumina NovaSeq 6000 platform with 151 bp paired-end reads.
- Linked publications:
- Center Name: Dr. Jitender Giri - National Institute of Plant Genome Research (NIPGR)
- Number of Base(Total) Mbp: 47,571,813,723
- Size in bytes(Total): 24,482,035,841
- Number of sample:
- Number of Runs:
- Number of Sequences:
- Number of Assembly:
- Number of Variant files:
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