IMDA — MS Study Report
Study ID: IMS_100076
Project ID: IMP_100076
Printed: 13 Nov 2026
Project
Project IDIMP_100076
TitleIntegrated Metabolite and Lipid Profiling of Human Vitreous Reveals Purine Metabolic Dysregulation and Inflammatory Lipid Remodeling as the Drivers of Bacterial Endophthalmitis
Uploaded byJoveeta
Research AreaBiological Sciences
Funding SourceHyderabad Eye Research Foundation
ContributorsJoveeta Joseph, Dhanwini Rudraprasad, Agimanailiu Khapuinamai, Sreyasi Maiti, Satyashree Gagan
DescriptionBacterial endophthalmitis, an intraocular infection and inflammation often progress rapidly and lead to irreversible vision loss especially in culture-negative cases where the diagnosis is delayed. We profiled infection-associated metabolomic and lipidomic alterations in the vitreous of patients with microbiological and clinically confirmed bacterial endophthalmitis to understand pathogenesis and identify distinct markers that drive infection and retinal injury.
Study
Study IDIMS_100076
TitleIntegrated Metabolite and Lipid Profiling of Human Vitreous Reveals Purine Metabolic Dysregulation and Inflammatory Lipid Remodeling as the Drivers of Bacterial Endophthalmitis.
Uploaded byJoveeta
Study TypeMass Spectrometry (MS)
Data TypeUntargeted
Release DateSept. 21, 2026
IEC/IBSC No.LEC-BHR-P-05-23-1045
Publication
SummaryBacterial endophthalmitis is a severe intraocular infection in which rapid inflammation, metabolic stress, and retinal injury can lead to irreversible vision loss. This study aimed to characterize infection-associated metabolic and lipid alterations in the human vitreous and identify biochemical signatures that may provide insight into disease pathogenesis and support culture-independent biomarker discovery. Undiluted vitreous samples were collected from 31 patients undergoing pars plana vitrectomy, including 16 patients with clinically and microbiologically confirmed bacterial endophthalmitis and 15 patients with non-infectious retinal conditions serving as controls. Samples were collected during surgery, processed rapidly, and stored at −80°C until analysis. Untargeted metabolomic and lipidomic profiling was performed using liquid chromatography–tandem mass spectrometry (LC–MS/MS), enabling broad characterization of small-molecule metabolites and lipid species. Following data normalization and statistical filtering, differential features were evaluated using multivariate analyses and pathway enrichment to identify biological processes associated with infection. The study revealed distinct metabolic and lipidomic profiles in infected vitreous, with alterations involving purine, amino acid, polyamine, redox, and vitamin metabolism, together with extensive remodeling of fatty acids, eicosanoids, bile acid derivatives, and membrane lipids. These findings provide a comprehensive view of the biochemical reprogramming associated with bacterial endophthalmitis and identify candidate molecular pathways for future diagnostic and therapeutic investigation.
Sample  (Total: 31)
# Sample ID Sample Name Organism Source Sample Type Experimental Condition Gender Age Replicates Storage Files/Sample
1 IMSM_105415 Control_1 Homo sapiens Human Vitreous Human Vitreous Control M 24 3 -80℃ 2
2 IMSM_105416 Control_2 Homo sapiens Human Vitreous Human Vitreous Control M 22 3 -80℃ 2
3 IMSM_105417 Control_3 Homo sapiens Human Vitreous Human Vitreous Control F 26 3 -80℃ 2
4 IMSM_105418 Control_4 Homo sapiens Human Vitreous Human Vitreous Control M 26 3 -80℃ 2
5 IMSM_105419 Control_5 Homo sapiens Human Vitreous Human Vitreous Control M 28 3 -80℃ 2
6 IMSM_105420 Control_6 Homo sapiens Human Vitreous Human Vitreous Control M 33 3 -80℃ 2
7 IMSM_105421 Control_7 Homo sapiens Human Vitreous Human Vitreous Control M 39 3 -80℃ 2
8 IMSM_105422 Control_8 Homo sapiens Human Vitreous Human Vitreous Control M 55 3 -80℃ 2
9 IMSM_105423 Control_9 Homo sapiens Human Vitreous Human Vitreous Control M 56 3 -80℃ 2
10 IMSM_105424 Control_10 Homo sapiens Human Vitreous Human Vitreous Control M 45 3 -80℃ 2
11 IMSM_105425 Control_11 Homo sapiens Human Vitreous Human Vitreous Control F 70 3 -80℃ 2
12 IMSM_105426 Control_12 Homo sapiens Human Vitreous Human Vitreous Control F 62 3 -80℃ 2
13 IMSM_105427 Control_13 Homo sapiens Human Vitreous Human Vitreous Control F 62 3 -80℃ 2
14 IMSM_105428 Control_14 Homo sapiens Human Vitreous Human Vitreous Control M 65 3 -80℃ 2
15 IMSM_105429 Control_15 Homo sapiens Human Vitreous Human Vitreous Control F 69 3 -80℃ 2
16 IMSM_105430 Infected_1 Homo sapiens Human Vitreous Human Vitreous Infected M 3 3 -80℃ 2
17 IMSM_105431 Infected_2 Homo sapiens Human Vitreous Human Vitreous Infected M 6 3 -80℃ 2
18 IMSM_105432 Infected_3 Homo sapiens Human Vitreous Human Vitreous Infected M 13 3 -80℃ 2
19 IMSM_105433 Infected_4 Homo sapiens Human Vitreous Human Vitreous Infected M 25 3 -80℃ 2
20 IMSM_105434 Infected_5 Homo sapiens Human Vitreous Human Vitreous Infected M 12 3 -80℃ 2
21 IMSM_105435 Infected_6 Homo sapiens Human Vitreous Human Vitreous Infected M 31 3 -80℃ 2
22 IMSM_105436 Infected_7 Homo sapiens Human Vitreous Human Vitreous Infected M 51 3 -80℃ 2
23 IMSM_105437 Infected_8 Homo sapiens Human Vitreous Human Vitreous Infected M 40 3 -80℃ 2
24 IMSM_105438 Infected_9 Homo sapiens Human Vitreous Human Vitreous Infected M 48 3 -80℃ 2
25 IMSM_105439 Infected_10 Homo sapiens Human Vitreous Human Vitreous Infected M 50 3 -80℃ 2
26 IMSM_105440 Infected_11 Homo sapiens Human Vitreous Human Vitreous Infected M 61 3 -80℃ 2
27 IMSM_105441 Infected_12 Homo sapiens Human Vitreous Human Vitreous Infected F 72 3 -80℃ 2
28 IMSM_105442 Infected_13 Homo sapiens Human Vitreous Human Vitreous Infected M 66 3 -80℃ 2
29 IMSM_105443 Infected_14 Homo sapiens Human Vitreous Human Vitreous Infected M 67 3 -80℃ 2
30 IMSM_105444 Infected_15 Homo sapiens Human Vitreous Human Vitreous Infected F 61 3 -80℃ 2
31 IMSM_105445 Infected_16 Homo sapiens Human Vitreous Human Vitreous Infected F 66 3 -80℃ 2
Mass Spectrometry Experiments
# MS Exp ID Sample Name / ID MS Type Instrument Name Instrument Type Ionization Method Ion Mode Software Used
1 IME_104553 Control_14 / IMSM_105428 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.19
2 IME_104526 Control_1 / IMSM_105415 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.6
3 IME_104527 Control_1 / IMSM_105415 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.6
4 IME_104528 Control_2 / IMSM_105416 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.7
5 IME_104529 Control_2 / IMSM_105416 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.7
6 IME_104530 Control_3 / IMSM_105417 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.8
7 IME_104531 Control_3 / IMSM_105417 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.8
8 IME_104532 Control_4 / IMSM_105418 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.9
9 IME_104533 Control_4 / IMSM_105418 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.9
10 IME_104534 Control_5 / IMSM_105419 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.10
11 IME_104535 Control_5 / IMSM_105419 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.10
12 IME_104536 Control_6 / IMSM_105420 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.11
13 IME_104537 Control_6 / IMSM_105420 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.11
14 IME_104538 Control_7 / IMSM_105421 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.12
15 IME_104539 Control_7 / IMSM_105421 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.12
16 IME_104540 Control_8 / IMSM_105422 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.13
17 IME_104541 Control_8 / IMSM_105422 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.13
18 IME_104542 Control_9 / IMSM_105423 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.14
19 IME_104543 Control_9 / IMSM_105423 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.14
20 IME_104544 Control_10 / IMSM_105424 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.15
21 IME_104545 Control_10 / IMSM_105424 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.15
22 IME_104546 Control_11 / IMSM_105425 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.16
23 IME_104547 Control_11 / IMSM_105425 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.16
24 IME_104548 Control_12 / IMSM_105426 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.17
25 IME_104549 Control_12 / IMSM_105426 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.17
26 IME_104550 Control_13 / IMSM_105427 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.18
27 IME_104551 Control_13 / IMSM_105427 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.18
28 IME_104552 Control_14 / IMSM_105428 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.19
29 IME_104554 Control_15 / IMSM_105429 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.20
30 IME_104555 Control_15 / IMSM_105429 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.20
31 IME_104556 Infected_1 / IMSM_105430 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.21
32 IME_104557 Infected_1 / IMSM_105430 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.21
33 IME_104558 Infected_2 / IMSM_105431 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.22
34 IME_104559 Infected_2 / IMSM_105431 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.22
35 IME_104560 Infected_3 / IMSM_105432 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.23
36 IME_104561 Infected_3 / IMSM_105432 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.23
37 IME_104562 Infected_4 / IMSM_105433 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.24
38 IME_104563 Infected_4 / IMSM_105433 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.24
39 IME_104564 Infected_5 / IMSM_105434 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.25
40 IME_104565 Infected_5 / IMSM_105434 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.25
41 IME_104566 Infected_6 / IMSM_105435 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.26
42 IME_104567 Infected_6 / IMSM_105435 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.26
43 IME_104568 Infected_7 / IMSM_105436 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.27
44 IME_104569 Infected_7 / IMSM_105436 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.27
45 IME_104570 Infected_8 / IMSM_105437 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.28
46 IME_104571 Infected_8 / IMSM_105437 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.28
47 IME_104572 Infected_9 / IMSM_105438 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.29
48 IME_104573 Infected_9 / IMSM_105438 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.29
49 IME_104574 Infected_10 / IMSM_105439 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.30
50 IME_104575 Infected_10 / IMSM_105439 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.30
51 IME_104576 Infected_11 / IMSM_105440 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.31
52 IME_104577 Infected_11 / IMSM_105440 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.31
53 IME_104578 Infected_12 / IMSM_105441 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.32
54 IME_104579 Infected_12 / IMSM_105441 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.32
55 IME_104580 Infected_13 / IMSM_105442 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.33
56 IME_104581 Infected_13 / IMSM_105442 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.33
57 IME_104582 Infected_14 / IMSM_105443 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.34
58 IME_104583 Infected_14 / IMSM_105443 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.34
59 IME_104584 Infected_15 / IMSM_105444 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.35
60 IME_104585 Infected_15 / IMSM_105444 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.35
61 IME_104586 Infected_16 / IMSM_105445 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.36
62 IME_104587 Infected_16 / IMSM_105445 Duo Spray Ion Source ABI Sciex 5500 QTrap Triple TOF Electrospray Ionization - ESI Positive;Negative Analyst TF 1.36
Author List
# First Name Last Name Email Organization Designation
1 Satyashree Gagan satyashree.gagan@gmail.com LV Prasad Eye Institute postdoctoral_researcher
2 Sreyasi Maiti sreyasideeni@gmail.com LV Prasad Eye Institute research_scholar
3 Agimanailiu Khapuinamai agimanailiu@gmail.com LV Prasad Eye Institute research_scholar
4 Dhanwini Rudraprasad satyashree.gagan@lvpei.org LV Prasad Eye Institute research_scholar
5 Joveeta Joseph joveeta@lvpei.org LV Prasad Eye Institute principal_investigator
File Details
# FTP Run ID MS Exp ID MS Data File
1 IMR_105152 IME_104548 Infection_P8.xlsx
2 IMR_105130 IME_104526 Control_P1.xlsx
3 IMR_105131 IME_104527 Control_P2.xlsx
4 IMR_105132 IME_104528 Control_P3.xlsx
5 IMR_105133 IME_104529 Control_P4.xlsx
6 IMR_105134 IME_104530 Control_P5.xlsx
7 IMR_105135 IME_104531 Control_P6.xlsx
8 IMR_105136 IME_104532 Control_P7.xlsx
9 IMR_105137 IME_104533 Control_P8.xlsx
10 IMR_105138 IME_104534 Control_P9.xlsx
11 IMR_105139 IME_104535 Control_P10.xlsx
12 IMR_105140 IME_104536 Control_P11.xlsx
13 IMR_105141 IME_104537 Control_P12.xlsx
14 IMR_105142 IME_104538 Control_P13.xlsx
15 IMR_105143 IME_104539 Control_P14.xlsx
16 IMR_105144 IME_104540 Control_P15.xlsx
17 IMR_105145 IME_104541 Infection_P1.xlsx
18 IMR_105146 IME_104542 Infection_P2.xlsx
19 IMR_105147 IME_104543 Infection_P3.xlsx
20 IMR_105148 IME_104544 Infection_P4.xlsx
21 IMR_105149 IME_104545 Infection_P5.xlsx
22 IMR_105150 IME_104546 Infection_P6.xlsx
23 IMR_105151 IME_104547 Infection_P7.xlsx
24 IMR_105153 IME_104549 Infection_P9.xlsx
25 IMR_105154 IME_104550 Infection_P10.xlsx
26 IMR_105155 IME_104551 Infection_P11.xlsx
27 IMR_105156 IME_104552 Infection_P12.xlsx
28 IMR_105157 IME_104553 Infection_P13.xlsx
29 IMR_105158 IME_104554 Infection_P14.xlsx
30 IMR_105159 IME_104555 Infection_P15.xlsx
31 IMR_105160 IME_104556 Infection_P16.xlsx
32 IMR_105161 IME_104557 Control_N1.xlsx
33 IMR_105162 IME_104558 Control_N2.xlsx
34 IMR_105163 IME_104559 Control_N3.xlsx
35 IMR_105164 IME_104560 Control_N4.xlsx
36 IMR_105165 IME_104561 Control_N5.xlsx
37 IMR_105166 IME_104562 Control_N6.xlsx
38 IMR_105167 IME_104563 Control_N7.xlsx
39 IMR_105168 IME_104564 Control_N8.xlsx
40 IMR_105169 IME_104565 Control_N9.xlsx
41 IMR_105170 IME_104566 Control_N10.xlsx
42 IMR_105171 IME_104567 Control_N11.xlsx
43 IMR_105172 IME_104568 Control_N12.xlsx
44 IMR_105173 IME_104569 Control_N13.xlsx
45 IMR_105174 IME_104570 Control_N14.xlsx
46 IMR_105175 IME_104571 Control_N15.xlsx
47 IMR_105176 IME_104572 Infection_N1.xlsx
48 IMR_105177 IME_104573 Infection_N2.xlsx
49 IMR_105178 IME_104574 Infection_N3.xlsx
50 IMR_105179 IME_104575 Infection_N4.xlsx
51 IMR_105180 IME_104576 Infection_N5.xlsx
52 IMR_105181 IME_104577 Infection_N6.xlsx
53 IMR_105182 IME_104578 Infection_N7.xlsx
54 IMR_105183 IME_104579 Infection_N8.xlsx
55 IMR_105184 IME_104580 Infection_N9.xlsx
56 IMR_105185 IME_104581 Infection_N10.xlsx
57 IMR_105186 IME_104582 Infection_N11.xlsx
58 IMR_105187 IME_104583 Infection_N12.xlsx
59 IMR_105188 IME_104584 Infection_N13.xlsx
60 IMR_105189 IME_104585 Infection_N14.xlsx
61 IMR_105190 IME_104586 Infection_N15.xlsx
62 IMR_105191 IME_104587 Infection_N16.xlsx
IMDA — Indian Metabolomics Data Archive  |  ibdc.rcb.res.in Study: IMS_100076  |  Project: IMP_100076