Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY4NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY5NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY6NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY7NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY8NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY9NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY10NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY11NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY12NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY13NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY14NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY15NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY16NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY17NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY18NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY19NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY20NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY21NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY22NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY23NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY24NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY25NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY26NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY27NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY28NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY29NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY30NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY31NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY32NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY33NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY34NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY35NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY36NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY37NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY38NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY39NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY40NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY41NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY42NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY43NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY44NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY45NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY46NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY47NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY48NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY49NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY50NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY51NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY52NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY53NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY54NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY55NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY56NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY57NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY58NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY59NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY60NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY61NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY62NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY63NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY64NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY65NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY66NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY67NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY68NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY69NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY70NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY71NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY72NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY73NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY74NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY75NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY76NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY77NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY78NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY79NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY80NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY81NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY82NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY83NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY84NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY85NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY86NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY87NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY88NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY89NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY90NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY91NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY92NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY93NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY94NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY95NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY96NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY97NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY98NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY99NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY100NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY101NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY102NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY103NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY104NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY105NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY106NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY107NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY108NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY109NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY110NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY111NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY112NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY113NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY114NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY115NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY116NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY117NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY118NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY119NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY120NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY121NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY122NN).

Microalgal biomass equivalent to 1 x 10⁷ cells was harvested from each culture flask via centrifugation. Intracellular metabolites were extracted by adding 1.0 mL of a chilled solvent mixture consisting of methanol, ethanol, and chloroform (1:3:1, v/v/v), supplemented with 3.0 μg/mL of 2-chloro-L-phenylalanine (Cayman Chemical Company) as an internal standard. Cell disruption was achieved by mechanical lysis using glass beads for 15 min, followed by centrifugation at 10,000 rpm, 4°C for 10 min to pellet the cellular debris. The resulting supernatant was collected and evaporated to dryness under vacuum at 4°C using a CentriVap centrifugal concentrator (Labconco). The dried metabolic residue was subsequently reconstituted in 200 μL of a resuspension mixture containing methanol, acetonitrile, and water (5:9:1, v/v/v). Prior to liquid chromatography-mass spectrometry (LC-MS) analysis, the resubmitted extract was clarified by filtration through a 0.2 μm syringe filter (MDI SY123NN).