| Project ID | IMP_100028 |
| Title | Multi-omics analysis of Aurantiochytrium limacinum |
| Uploaded by | Pannaga Pavan |
| Research Area | Biological Sciences |
| Funding Source | Department of Biotechnology (DBT) |
| Contributors | Pannaga Pavan Jutur |
| Description | To evaluate temporal changes in the presence of glycerol, time-course metabolite profiling was done in the presence of glycerol and glucose at three different time-points i.e., 0, 48 and 96 h using GCMS. |
| Study ID | IMS_100022 |
| Title | Time course GCMS based untargeted metabolomics in the presence of glucose and glycerol |
| Uploaded by | Pannaga Pavan |
| Study Type | Mass Spectrometry (MS) |
| Data Type | Untargeted |
| Release Date | March 6, 2025 |
| IEC/IBSC No. | |
| Publication | |
| Summary | Our preliminary analysis identifies that glycerol enhanced DHA accumulation in native isolate Aurantiochytrium limacinum. To evaluate temporal changes in the presence of glycerol, time-course metabolite profiling was done in the presence of glycerol and glucose at three different time-points i.e., 0, 48 and 96 h using GC-MS. We identified nearly 40 metabolites, among which metabolites belonging to pentose phosphate pathway, TCA cycle and amino acid metabolism were significantly differentially regulated which suggests its role in glycerol induced DHA accumulation. |
| # | Sample ID | Sample Name | Organism | Source | Sample Type | Experimental Condition | Gender | Age | Replicates | Storage | Files/Sample |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | IMSM_101065 | D0_1 | Aurantiochytrium limacinum | Cultured cells | Cultured cells | Glucose (3% w/v) in YPD medium | NA | NA | NA | NA | |
| 2 | IMSM_101066 | D4_Glucose_1 | Aurantiochytrium limacinum | Cultured cells | Cultured cells | Glucose (3% w/v) in YPD medium | NA | NA | NA | NA | |
| 3 | IMSM_101067 | D4_Glucose_2 | Aurantiochytrium limacinum | Cultured cells | Cultured cells | Glucose (3% w/v) in YPD medium | NA | NA | NA | NA | |
| 4 | IMSM_101068 | D4_Glucose_3 | Aurantiochytrium limacinum | Cultured cells | Cultured cells | Glucose (3% w/v) in YPD medium | NA | NA | NA | NA | |
| 5 | IMSM_101069 | D2_Glucose_3 | Aurantiochytrium limacinum | Cultured cells | Cultured cells | Glucose (3% w/v) in YPD medium | NA | NA | NA | NA | |
| 6 | IMSM_101070 | D2_Glucose_1 | Aurantiochytrium limacinum | Cultured cells | Cultured cells | Glucose (3% w/v) in YPD medium | NA | NA | NA | NA | |
| 7 | IMSM_101071 | D0_2 | Aurantiochytrium limacinum | Cultured cells | Cultured cells | Glucose (3% w/v) in YPD medium | NA | NA | NA | NA | |
| 8 | IMSM_101072 | D2_Glucose_2 | Aurantiochytrium limacinum | Cultured cells | Cultured cells | Glucose (3% w/v) in YPD medium | NA | NA | NA | NA | |
| 9 | IMSM_101073 | D0_3 | Aurantiochytrium limacinum | Cultured cells | Cultured cells | Glucose (3% w/v) in YPD medium | NA | NA | NA | NA | |
| 10 | IMSM_101074 | D4_Glycerol_3 | Aurantiochytrium limacinum | Cultured cells | Cultured cells | Glycerol (3% w/v) in YPD medium | NA | NA | NA | NA | |
| 11 | IMSM_101075 | D4_Glycerol_2 | Aurantiochytrium limacinum | Cultured cells | Cultured cells | Glycerol (3% w/v) in YPD medium | NA | NA | NA | NA | |
| 12 | IMSM_101076 | D2_Glycerol_2 | Aurantiochytrium limacinum | Cultured cells | Cultured cells | Glycerol (3% w/v) in YPD medium | NA | NA | NA | NA | |
| 13 | IMSM_101077 | D2_Glycerol_1 | Aurantiochytrium limacinum | Cultured cells | Cultured cells | Glycerol (3% w/v) in YPD medium | NA | NA | NA | NA | |
| 14 | IMSM_101078 | D2_Glycerol_3 | Aurantiochytrium limacinum | Cultured cells | Cultured cells | Glycerol (3% w/v) in YPD medium | NA | NA | NA | NA | |
| 15 | IMSM_101079 | D4_Glycerol_1 | Aurantiochytrium limacinum | Cultured cells | Cultured cells | Glycerol (3% w/v) in YPD medium | NA | NA | NA | NA |
| # | MS Exp ID | Sample Name / ID | MS Type | Instrument Name | Instrument Type | Ionization Method | Ion Mode | Software Used |
|---|---|---|---|---|---|---|---|---|
| 1 | IME_100539 | D0_1 / IMSM_101065 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| 2 | IME_100540 | D4_Glucose_1 / IMSM_101066 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| 3 | IME_100541 | D4_Glucose_2 / IMSM_101067 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| 4 | IME_100542 | D4_Glucose_3 / IMSM_101068 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| 5 | IME_100543 | D2_Glucose_3 / IMSM_101069 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| 6 | IME_100544 | D2_Glucose_1 / IMSM_101070 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| 7 | IME_100545 | D0_2 / IMSM_101071 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| 8 | IME_100546 | D2_Glucose_2 / IMSM_101072 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| 9 | IME_100547 | D0_3 / IMSM_101073 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| 10 | IME_100548 | D4_Glycerol_3 / IMSM_101074 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| 11 | IME_100549 | D4_Glycerol_2 / IMSM_101075 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| 12 | IME_100550 | D2_Glycerol_2 / IMSM_101076 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| 13 | IME_100551 | D2_Glycerol_1 / IMSM_101077 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| 14 | IME_100552 | D2_Glycerol_3 / IMSM_101078 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| 15 | IME_100553 | D4_Glycerol_1 / IMSM_101079 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| # | First Name | Last Name | Organization | Designation | |
|---|---|---|---|---|---|
| 1 | Pannaga Pavan | Jutur | jppavan@icgeb.res.in | International Centre for Genetic Engineering and Biotechnology | principal_investigator |
| # | FTP Run ID | MS Exp ID | MS Data File |
|---|---|---|---|
| 1 | IMR_101138 | IME_100539 | D0_1.mzdata.xml |
| 2 | IMR_101139 | IME_100540 | D4_GLU_1.mzdata.xml |
| 3 | IMR_101140 | IME_100541 | D4_GLU_2.mzdata.xml |
| 4 | IMR_101141 | IME_100542 | D4_GLU_3.mzdata.xml |
| 5 | IMR_101142 | IME_100543 | D2_GLU_3.mzdata.xml |
| 6 | IMR_101143 | IME_100544 | D2_GLU_1.mzdata.xml |
| 7 | IMR_101144 | IME_100545 | D0_2.mzdata.xml |
| 8 | IMR_101145 | IME_100546 | D2_GLU_2.mzdata.xml |
| 9 | IMR_101146 | IME_100547 | D0_3.mzdata.xml |
| 10 | IMR_101147 | IME_100548 | D4_GLY_3.mzdata.xml |
| 11 | IMR_101148 | IME_100549 | D4_GLY_2.mzdata.xml |
| 12 | IMR_101149 | IME_100550 | D2_GLY_2.mzdata.xml |
| 13 | IMR_101150 | IME_100551 | D2_GLY_1.mzdata.xml |
| 14 | IMR_101151 | IME_100552 | D2_GLY_3.mzdata.xml |
| 15 | IMR_101152 | IME_100553 | D4_GLY_1.mzdata.xml |