| Project ID | IMP_100022 |
| Title | Metabolite profiling of MNSM by LC-MS/MS |
| Uploaded by | Rajib |
| Research Area | Biological Sciences |
| Funding Source | ICGEB, New Delhi |
| Contributors | ICGEB, New Delhi and UGC, Nepal |
| Description | LC-MS/MS analysis of Methanol extract of Mahonia napaulensis DC. Stem (MNSM) |
| Study ID | IMS_100017 |
| Title | Metabolite profiling of MNSM by LC-MS/MS |
| Uploaded by | Rajib |
| Study Type | Mass Spectrometry (MS) |
| Data Type | Untargeted |
| Release Date | Feb. 13, 2025 |
| IEC/IBSC No. | |
| Publication | |
| Summary | The metabolite profiling of methanol extract of bark of M. napaulensis (MNBM) was done by LC-MS/MS analysis. The plant stem was cleaned and shade dried. The dried plant stem was ground into fine powder. The extraction was done by cold maceration process. The extract was filtered using Whatman filter paper No. 1. and was dried under reduced pressure at room temperature below 40oC, the extract was stored in closed vials at 4oC for further analysis. The metabolite profiling of methanol extract of M. napaulensis stem (MNSM) was done by LC-MS/MS analysis. The LC-MS/MS analysis identified 115 metabolites. The most abundant five metabolites are - dihydroberberine (86.05%), D-(+)-malic acid (3.43%), D-(-)-quinic acid (2.71%),(2R)-3-{[(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-{[10-(2-hexylcyclopropyl)decanoyl] oxy}propyl palmitate (2.11%) and D-(−)-erythrose (0.83%). The literature study through PubChem and Chemical Entities of Biological Interest (ChEBI) reported that the metabolites dihydroberberine (86.05%), curcumin (0.49%), protopine (0.40%), hymecromone (0.07%), a-linolenic Acid (0.06%), menadione (0.06%), 6-gingerol (0.04%), (S)-equol (0.03%), pterostilbene (0.004%), phloretin (0.002%), 1-O-feruloyl-β-D-glucose (0.002%), and naringin (0.002%) have anticancer activity. |
| # | Sample ID | Sample Name | Organism | Source | Sample Type | Experimental Condition | Gender | Age | Replicates | Storage | Files/Sample |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | IMSM_100995 | M. napaulensis Stem | Mahonia nepalensis | Plant-stem | Plant Sample | Methanol Extract of stem of M. napaulensis | NA | NA | NA | 4oC | 1 |
| 2 | IMSM_100996 | M. napaulensis Stem | Mahonia nepalensis | Plant-stem | Plant Sample(02) | Methanol Extract of stem of M. napaulensis | NA | NA | NA | 4oC | 1 |
| # | MS Exp ID | Sample Name / ID | MS Type | Instrument Name | Instrument Type | Ionization Method | Ion Mode | Software Used |
|---|---|---|---|---|---|---|---|---|
| 1 | IME_100504 | M. napaulensis Stem / IMSM_100996 | LCMS (Liquid Chromatography- Mass Spectrometry) | Thermo Q Exactive Orbitrap | Orbitrap | Others | Negative | Compound Discoverer |
| 2 | IME_100505 | M. napaulensis Stem / IMSM_100995 | LCMS (Liquid Chromatography- Mass Spectrometry) | Thermo Q Exactive Orbitrap | Orbitrap | Others | Positive | Compound Discoverer |
| # | First Name | Last Name | Organization | Designation | |
|---|---|---|---|---|---|
| 1 | Dr Rajib Kumar | Shrestha | shrestharajib2005@ku.edu.np | Dept. of Chemical Science and Engineering School of Engineering, Kathmandu University Dhulikhel, Kavre, Nepal | scientist |
| # | FTP Run ID | MS Exp ID | MS Data File |
|---|---|---|---|
| 1 | IMR_101103 | IME_100504 | M_napaulensis_stem_N.raw |
| 2 | IMR_101104 | IME_100505 | M_napaulensis_stem_P.raw |