| Project ID | IMP_100015 |
| Title | Metabolite profiling of MNBM by LC-MS/MS |
| Uploaded by | Rajib |
| Research Area | Biological Sciences |
| Funding Source | ICGEB, New Delhi |
| Contributors | ICGEB, New Delhi and UGC, Nepal |
| Description | Metabolite profiling of Mahonia napaulensis bark methanol extract (MNBM) of Nepal by LC-MS/MS |
| Study ID | IMS_100012 |
| Title | LC-MS/MS analysis of chloroform fraction (MNSMC) of methanol extract of stem of M. napaulensis. |
| Uploaded by | Rajib |
| Study Type | Mass Spectrometry (MS) |
| Data Type | Untargeted |
| Release Date | Dec. 20, 2024 |
| IEC/IBSC No. | |
| Publication | |
| Summary | Metabolite profiling of most active chloroform fraction (MNSMC) by LC-MS/MS analysis Since chloroform fraction of methanol extract of M. napaulensis stem was the most active fraction, its metabolite profiling was done by LC-MS/MS analysis. The LC-MS/MS analysis identified 153 metabolites. The most abundant five metabolites are cetrimonium (36.82 %), berberine (11.14 %), (15Z)-9,12,13-Trihydroxy-15-octadecenoic acid (7.31 %), protopine (6.13%) and (5R,6S)-5-Hydroxy-4-methoxy-6-(2-phenylethyl)-5,6-dihydro-2H-pyran-2-one (3.31 %). The literature study through PubChem and Chemical Entities of Biological Interest (ChEBI) reported that the metabolites berberine (11.14 %), protopine (6.13%), luteolin (1.07%), apigenin (0.64%), menadione (0.29%), tangeretin (0.21%), matairesinol (0.16%), dioscin (0.15%), nobiletin (0.14%), (E)-dacarbazine (0.12%), salirasib (0.11%), NECA (0.03%), MTIC (0.02%), mitoxantrone (0.01%), hydroxytyrosol (0.01%), and betulin (0.01%) have anticancer activity. |
| # | Sample ID | Sample Name | Organism | Source | Sample Type | Experimental Condition | Gender | Age | Replicates | Storage | Files/Sample |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | IMSM_100841 | M. napaulensis Stem | Mahonia nepalensis | Plant-stem | Plant Sample | Chloroform fraction (MNSMC) of methanol extract of stem of Mahonia napaulensis | NA | NA | NA | 4oC | |
| 2 | IMSM_100842 | M. napaulensis Stem | Mahonia nepalensis | Plant-stem | Plant Sample (02) | Chloroform fraction (MNSMC) of methanol extract of stem of Mahonia napaulensis | NA | NA | NA | 4oC |
| # | MS Exp ID | Sample Name / ID | MS Type | Instrument Name | Instrument Type | Ionization Method | Ion Mode | Software Used |
|---|---|---|---|---|---|---|---|---|
| 1 | IME_100490 | M. napaulensis Stem / IMSM_100841 | LCMS (Liquid Chromatography- Mass Spectrometry) | Thermo Q Exactive Plus Orbitarp | Orbitrap | Others | Positive | Compound Discoverer |
| 2 | IME_100491 | M. napaulensis Stem / IMSM_100842 | LCMS (Liquid Chromatography- Mass Spectrometry) | Thermo Q Exactive Plus Orbitarp | Orbitrap | Others | Negative | Compound Discoverer |
| # | First Name | Last Name | Organization | Designation | |
|---|---|---|---|---|---|
| 1 | Rajib | Kumar Shrestha | shrestharajib2005@ku.edu.np | Kathmandu University, Nepal | scientist |
| # | FTP Run ID | MS Exp ID | MS Data File |
|---|---|---|---|
| 1 | IMR_101089 | IME_100490 | Mahonia_napaulensis_Stem_P.raw |
| 2 | IMR_101090 | IME_100491 | Mahonia_napaulensis_Stem_N.raw |