| Project ID | IMP_100015 |
| Title | Metabolite profiling of MNBM by LC-MS/MS |
| Uploaded by | Rajib |
| Research Area | Biological Sciences |
| Funding Source | ICGEB, New Delhi |
| Contributors | ICGEB, New Delhi and UGC, Nepal |
| Description | Metabolite profiling of Mahonia napaulensis bark methanol extract (MNBM) of Nepal by LC-MS/MS |
| Study ID | IMS_100011 |
| Title | Metabolite profiling of MNBM by LC-MS/MS |
| Uploaded by | Rajib |
| Study Type | Mass Spectrometry (MS) |
| Data Type | Untargeted |
| Release Date | Dec. 20, 2024 |
| IEC/IBSC No. | |
| Publication | http://doi.org/10.1186/s12906-025-04806-0 |
| Summary | The liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis was performed in VProteomics Laboratory, New Delhi, India. The metabolite profiling of methanol extract of bark of M. napaulensis (MNBM) was done by LC-MS/MS analysis and it identified 507 compounds. The chemical contents, retention time and area percentage of most abundant 59 metabolites are sorted according to maximum to minimum abundance order. The most abundant five metabolites are dihydroberberine (34.25%), ethylmorphine (8.91%), 2-(Methylthiomethyl)-3-phenylacrylaldehyde (8.57%), ochotensine (7.29%) and d-berbamine (6.56%). The literature study through PubChem and Chemical Entities of Biological Interest (ChEBI) reported that the metabolites dihydroberberine (34.25%), d-berbamine (6.56 %), (S)-glaucine (1.02%), protopine (0.54%), grosheimin (0.36%), mycophenolic acid (0.18%), 5-O-methylembelin (0.14%), berberine (0.12%), (-)-(S)-equol (0.11%), alpha-linolenic acid (0.09%), polidocanol (0.08%), juglone (0.07%), lapachol (0.05%), solasodine (0.05%), podophyllotoxin (0.05%), tandutinib (0.04%), mitoxantrone (0.03%), methyl 3-indolylacetate (0.03%), eugenol (0.02%), apaziquone (0.02%) and hymecromone (0.01%) have anticancer activity. |
| # | Sample ID | Sample Name | Organism | Source | Sample Type | Experimental Condition | Gender | Age | Replicates | Storage | Files/Sample |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | IMSM_100835 | M. napaulensis Bark | Mahonia nepalensis | Plant-stem | Plant Sample | Methanol Extract of bark of M. napaulensis | NA | NA | NA | 4oC | 1 |
| 2 | IMSM_100840 | M. napaulensis Bark | Mahonia nepalensis | Plant-stem | Plant Sample (02) | Methanol Extract of Bark of M. napaulensis | NA | NA | NA | 4oC | 1 |
| # | MS Exp ID | Sample Name / ID | MS Type | Instrument Name | Instrument Type | Ionization Method | Ion Mode | Software Used |
|---|---|---|---|---|---|---|---|---|
| 1 | IME_100488 | M. napaulensis Bark / IMSM_100835 | Thermo Exploris 240D | Others | Orbitrap | Others | Positive | Compound Discoverer |
| 2 | IME_100489 | M. napaulensis Bark / IMSM_100840 | Thermo Exploris 240D | Others | Orbitrap | Others | Negative | Compound Discoverer |
| # | First Name | Last Name | Organization | Designation | |
|---|---|---|---|---|---|
| 1 | Rajib | Kumar Shrestha | shrestharajib2005@ku.edu.np | Kathmandu University, Nepal | research_scholar |
| # | FTP Run ID | MS Exp ID | MS Data File |
|---|---|---|---|
| 1 | IMR_101087 | IME_100488 | M_napaulensis_Bark_P.raw |
| 2 | IMR_101088 | IME_100489 | M_napaulensis_Bark_N.raw |