Study Data


MS Study

Project uploaded by: Vineet K.
Project ID: IMP_100008
Title: The unique composition of Indian gut microbiome, gene catalogue and associated faecal metabolome deciphered using multi-omics approaches
Project Description: Metagenomic studies carried out in the past decade have led to an enhanced understanding of the gut microbiome in human health, however, the Indian gut microbiome is not well explored yet. We analysed the gut microbiome of 110 healthy individuals from two distinct locations (North-Central and Southern) in India using multi-omics approaches, including 16S rRNA gene amplicon sequencing, whole genome shotgun metagenomic sequencing, and metabolomic profiling of faecal and serum samples.
Research Area: Biological Sciences
Funding Source: Intramural funds from IISER Bhopal
Project Contributors: Darshan B. Dhakan, Abhijit Maji, Rituja Saxena, Vineet K. Sharma

Study uploaded by: Vineet K.
Study ID: IMS_100008
Title: The unique composition of Indian gut microbiome, gene catalogue and associated faecal metabolome deciphered using multi-omics approaches
Summary: Metagenomic studies carried out in the past decade have led to an enhanced understanding of the gut microbiome in human health, however, the Indian gut microbiome is not well explored yet. We analysed the gut microbiome of 110 healthy individuals from two distinct locations (North-Central and Southern) in India using multi-omics approaches, including 16S rRNA gene amplicon sequencing, whole genome shotgun metagenomic sequencing, and metabolomic profiling of faecal and serum samples.
Publication: https://doi.org/10.1093/gigascience/giz004
Release Date: May 14, 2024
Study Type: Mass Spectrometry (MS)
Data Type: Untargeted
IEC/IBSC Approval Number :

Sr.No Sample ID Sample Name Organism Source Sample Preparation Protocol Sample Type Experimental Condition Time of treatment Variant/Variety Gender Age Replicates Storage Conditions Extraction Protocol Number of files per sample
1 IMSM_100201 HA Homo sapiens | 9606 Feces N/A Healthy NA 26 NA NA

NA
2 IMSM_100310 HAA Homo sapiens | 9606 Feces N/A Healthy NA 27 NA NA

NA
3 IMSM_100311 HAB Homo sapiens | 9606 Feces N/A Healthy NA 28 NA NA

NA
4 IMSM_100312 HAC Homo sapiens | 9606 Feces N/A Healthy NA 21 NA NA

NA
5 IMSM_100313 HAD Homo sapiens | 9606 Feces N/A Healthy NA 27 NA NA

NA
6 IMSM_100314 HAE Homo sapiens | 9606 Feces N/A Healthy NA 21 NA NA

NA
7 IMSM_100315 HAF Homo sapiens | 9606 Feces N/A Healthy NA 21 NA NA

NA
8 IMSM_100316 HAG Homo sapiens | 9606 Feces N/A Healthy NA 23 NA NA

NA
9 IMSM_100317 HAH Homo sapiens | 9606 Feces N/A Healthy NA 25 NA NA

NA
10 IMSM_100318 HAI Homo sapiens | 9606 Feces N/A Healthy NA 24 NA NA

NA

Sr.No MS Exp ID Sample Name/ID Mass Spectrometer Type MS Instrument Name MS Instrument type MS Ionization Method Ion Mode/Scan Polarity Data Transformation (Software/s Used)
21 IME_100287 HB_S / IMSM_100429 GCMS (Gas Chromatography -Mass Spectrometry) Agilent 5975C Single quadrupole Electron Ionization - EI Positive N/A
22 IME_100288 HAT_S / IMSM_100428 GCMS (Gas Chromatography -Mass Spectrometry) Agilent 5975C Single quadrupole Electron Ionization - EI Positive N/A
23 IME_100289 HAR_S / IMSM_100427 GCMS (Gas Chromatography -Mass Spectrometry) Agilent 5975C Single quadrupole Electron Ionization - EI Positive N/A
24 IME_100290 HAP_S / IMSM_100426 GCMS (Gas Chromatography -Mass Spectrometry) Agilent 5975C Single quadrupole Electron Ionization - EI Positive N/A
25 IME_100291 HAI_S / IMSM_100425 GCMS (Gas Chromatography -Mass Spectrometry) Agilent 5975C Single quadrupole Electron Ionization - EI Positive N/A
26 IME_100292 HAH_S / IMSM_100424 GCMS (Gas Chromatography -Mass Spectrometry) Agilent 5975C Single quadrupole Electron Ionization - EI Positive N/A
27 IME_100293 HAG_S / IMSM_100423 GCMS (Gas Chromatography -Mass Spectrometry) Agilent 5975C Single quadrupole Electron Ionization - EI Positive N/A
28 IME_100294 HAF_S / IMSM_100422 GCMS (Gas Chromatography -Mass Spectrometry) Agilent 5975C Single quadrupole Electron Ionization - EI Positive N/A
29 IME_100295 HAE_S / IMSM_100421 GCMS (Gas Chromatography -Mass Spectrometry) Agilent 5975C Single quadrupole Electron Ionization - EI Positive N/A
30 IME_100296 HAD_S / IMSM_100420 GCMS (Gas Chromatography -Mass Spectrometry) Agilent 5975C Single quadrupole Electron Ionization - EI Positive N/A

Sr.No First name Last name Email Organization Designation
1 Vineet K. Sharma vineetks@iiserb.ac.in Indian Institute of Science Education and Research, Bhopal principal_investigator
2 Rituja Saxena ritujaritu@gmail.com Indian Institute of Science Education and Research, Bhopal research_scholar
3 Abhijit Maji abhi71084@gmail.com Indian Institute of Science Education and Research, Bhopal postdoctoral_researcher
4 Darshan B. Dhakan dardhakkan@gmail.com Indian Institute of Science Education and Research, Bhopal research_scholar

Sr.No ftprun ID MS Exp ID MS Data Files
61 IMR_100926 IME_100327 SAMN07449066.CDF
62 IMR_100927 IME_100328 SAMN07449065.CDF
63 IMR_100928 IME_100329 SAMN07449064.CDF
64 IMR_100929 IME_100330 SAMN07449061.CDF
65 IMR_100930 IME_100331 SAMN07449060.CDF
66 IMR_100931 IME_100332 SAMN07449059.CDF
67 IMR_100932 IME_100333 SAMN07449058.CDF
68 IMR_100933 IME_100334 SAMN07449057.CDF
69 IMR_100934 IME_100335 SAMN07449056.CDF
70 IMR_100935 IME_100336 SAMN07449055.CDF

Download Metabolite/Compound

Sr.No Structure Details
1 Molecular Structure

Glycine

CHEBI ID: CHEBI:15428

PubChem ID: 750

Chemical Formula: C2H5NO2

Smiles: NCC(O)=O

InChI: InChI=1S/C2H5NO2/c3-1-2(4)5/h1,3H2,(H,4,5)

Mass to charge: 174.1

Retention Time: 536
2 Molecular Structure

Mannose

CHEBI ID: CHEBI:37684

PubChem ID: 18950

Chemical Formula: C6H12O6

Smiles: C([C@@H]1[C@H]([C@@H]([C@@H](C(O1)O)O)O)O)O

InChI: InChI=1S/C6H12O6/c7-1-2-3(8)4(9)5(10)6(11)12-2/h2-11H,1H2/t2-,3-,4+,5+,6?/m1/s1

Mass to charge: 173.0

Retention Time: 976
3 Molecular Structure

Glucose

CHEBI ID: CHEBI:17234

PubChem ID: 5793

Chemical Formula: C6H12O6

Smiles: C([C@@H]1[C@H]([C@@H]([C@H](C(O1)O)O)O)O)O

InChI: InChI=1S/C6H12O6/c7-1-2-3(8)4(9)5(10)6(11)12-2/h2-11H,1H2/t2-,3-,4+,5-,6?/m1/s1

Mass to charge: 173.0

Retention Time: 976
4 Molecular Structure

Palmitic acid

CHEBI ID: CHEBI:15756

PubChem ID: 985

Chemical Formula: C16H32O2

Smiles: CCCCCCCCCCCCCCCC(O)=O

InChI: InChI=1S/C16H32O2/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16(17)18/h2-15H2,1H3,(H,17,18)

Mass to charge: 313.2

Retention Time: 1045
5 Molecular Structure

Stearic acid

CHEBI ID: CHEBI:28842

PubChem ID: 5281

Chemical Formula: C18H36O2

Smiles: C(CCCCCCCCCC)CCCCCCC(=O)O

InChI: InChI=1S/C18H36O2/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18(19)20/h2-17H2,1H3,(H,19,20)

Mass to charge: 117.0

Retention Time: 1155
6 Molecular Structure

Valeric acid

CHEBI ID: CHEBI:17418

PubChem ID: 10449

Chemical Formula: C5H10O2

Smiles: CCCCC(O)=O

InChI: InChI=1S/C5H10O2/c1-2-3-4-5(6)7/h2-4H2,1H3,(H,6,7)

Mass to charge: 159.1

Retention Time: 268
7 Molecular Structure

beta-Hydroxy butyric acid

CHEBI ID: CHEBI:20067

PubChem ID: 441

Chemical Formula: C4H8O3

Smiles: CC(O)CC(O)=O

InChI: InChI=1S/C4H8O3/c1-3(5)2-4(6)7/h3,5H,2H2,1H3,(H,6,7)

Mass to charge: 117.1

Retention Time: 328
8 Molecular Structure

Alanine

CHEBI ID: CHEBI:16449

PubChem ID: 5950

Chemical Formula: C3H7NO2

Smiles: CC(N)C(O)=O

InChI: InChI=1S/C3H7NO2/c1-2(4)3(5)6/h2H,4H2,1H3,(H,5,6)

Mass to charge: 101.8

Retention Time: 363
9 Molecular Structure

Valine

CHEBI ID: CHEBI:27266

PubChem ID: 6287

Chemical Formula: C5H11NO2

Smiles: CC(C)C(N)C(O)=O

InChI: InChI=1S/C5H11NO2/c1-3(2)4(6)5(7)8/h3-4H,6H2,1-2H3,(H,7,8)

Mass to charge: 144.1

Retention Time: 458
10 Molecular Structure

L-Leucine

CHEBI ID: CHEBI:15603

PubChem ID: 6106

Chemical Formula: C6H13NO2

Smiles: CC(C)C[C@H](N)C(O)=O

InChI: InChI=1S/C6H13NO2/c1-4(2)3-5(7)6(8)9/h4-5H,3,7H2,1-2H3,(H,8,9)/t5-/m0/s1

Mass to charge: 159.1

Retention Time: 505
11 Molecular Structure

L-isoleucine

CHEBI ID: CHEBI:17191

PubChem ID: 6306

Chemical Formula: C6H13NO2

Smiles: CC[C@H](C)[C@H](N)C(O)=O

InChI: InChI=1S/C6H13NO2/c1-3-4(2)5(7)6(8)9/h4-5H,3,7H2,1-2H3,(H,8,9)/t4-,5-/m0/s1

Mass to charge: 158.1

Retention Time: 524