| Study ID | PST_100069 |
| Study Title | Evaluation of Maximum Quantum Yield of PSII in Wild type PB1 and overexpressing MIR408 plants |
| Description | Maximum quantum yield of PSII (Fv/Fm) was measured in one month old WT and MIR408 overexpressing plants using a Pulse-amplitude modulation fluorometer (Junior-PAM, H.Waltz, Germany). Plants were dark adapted for 30 min before measuring maximum photosynthetic efficiency (Fv/Fm). The electron transport rate (ETR) and effective photosynthetic efficiency (YII) were recorded in light adapted plants. More than 10 plants were used for each reading. |
| Crop | Oryza sativa |
| Meta-Trait | Maximum quantum yield of PSII |
| Data Type | Phenotypic |
| Location | DPMB-UDSC, New Delhi |
| Study Period | 03 Jul 2014 – 20 Jul 2014 |
| Growth Facility | Culture room |
| Experimental Design | The wild type (WT) and MIR408-OE seeds were sterilized with 70% ethanol and then with 0.1% HgCl2, having a few drops of Tween-20 with shaking for 15 min. They were then washed with reverse osmosis (RO) water several times and soaked in RO water overnight in the dark. The next day, the seeds were spread on a cotton bed in small plastic trays evenly moistened with RO water. Trays were covered with a clean film and allowed to grow at 28 °C ± 2 in culture room conditions with a photocycle of 14 h light and 10 h dark for 1 week in rice growth medium (RGM; Yoshida et al., 1976). One-week seedlings were transferred to test tubes and grown hydroponically under culture room conditions (temperature = 28 °C ± 2 with a photocycle of 14 h light and 10 h dark; 60–75 % relative humidity). The miR408 overexpressing plants and WT plants were grown in rice growth room conditions for one month and 16 plants from each set was used for the evaluation of Maximum quantum yield of PSII (Fv/Fm) |
| Tolerant Check | -- |
| Susceptible Check | -- |
| Uploaded By | Saurabh Raghuvanshi |
| Created | 27 Jan 2025 |
Indian Crop Phenome Database