| Study ID | PST_100011 |
| Study Title | Characterization of vegetative vigor of miR408 overexpression and wild type plants. |
| Description | The T2 generation of miR408 overexpression plants along with WT plants compared for different traits to characterize the role of miR408 in growth and development. Phenotypic comparison of 40-day-old wild type and MIR408-OE plants were conducted in culture room conditions. Quantitative analysis of shoot and root length, root density, plant weight, leaf blade lengthand leaf blade width were studied. |
| Crop | Oryza sativa |
| Meta-Trait | vegetative vigor |
| Data Type | Phenotypic |
| Location | New Delhi |
| Study Period | 01 Aug 2014 – 10 Sep 2014 |
| Growth Facility | culture room |
| Experimental Design | The wild type (WT) and MIR408-OE seeds were sterilized with 70% ethanol and then with 0.1% HgCl2, having a few drops of Tween-20 with shaking for 15 min. They were then washed with reverse osmosis (RO) water several times and soaked in RO water overnight in the dark. The next day, the seeds were spread on a cotton bed in small plastic trays evenly moistened with RO water. Trays were covered with a clean film and allowed to grow at 28 °C ± 2 in culture room conditions with a photocycle of 14 h light and 10 h dark for 1 week in rice growth medium (RGM; Yoshida et al., 1976). One-week seedlings were transferred to test tubes and grown hydroponically under culture room conditions (temperature = 28 °C ± 2 with a photocycle of 14 h light and 10 h dark; 60–75 % relative humidity). The transgenic lines were analyzed for their morphometric differences from the wild-type plants. The shoot length, root length, root density, 3rd leaf length and width (from the midpoint), and whole plant weight at different stages (15-day-old; 40-day-old) were recorded for both transgenic lines and wild-type plants. For most of the lines, more than 10 plants were analyzed for each parameter. |
| Tolerant Check | -- |
| Susceptible Check | -- |
| Uploaded By | Saurabh Raghuvanshi |
| Created | 27 Sep 2023 |
Indian Crop Phenome Database